CV-HLVD-LCV Multiplex RT-PCR Detection Kit is used for the detection of cryptic virus (CV), hop latent viroid(HLVD), and lettuce chlorosis (LCV) from the cannabis plant in one step RT-PCR reaction. It combines both reverse transcription (RT) and Taqman probe-based PCR amplification to occur in a single reaction tube. The kit is optimized for the two reactions in a real-time “single step”. This One-step qRT-PCR assay offers the end-users an efficient, easy-to-use, and reliable alternative to conventional “two-step” sequential qRT-PCR for detecting CV, HLVd, and LCV from plant samples.
Tribioscience Multiplex RT-PCR Detection Kit contains all components for RT-PCR reaction including RT-PCR enzymes, primer probes, internal control, positive control, negative control, and buffer. The 3 target genes are labeled with a specific fluorescence color: Cy5 for Cryptic virus, Fam for HLVD, ROX for lettuce chlorosis virus, and Hex for internal control.
Applications
This kit is used for the detection of CV, HLVd, and LCV infection in plant samples.
Key Features
One-step complete qRT-PCR in a single tube.
Reduce contamination in the operating process.
Accurate detection and quantification of HLVd target gene.
4x RT-PCR mix makes it easier to adjust the sample size in a one-tube reaction.
Storage Conditions
Store all components at -20°C in a non-frost-free freezer.
Shelf life is 12 months after receipt. The kit is shipped on ice.
