Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅢ 10U/mg-solid or more (containing approx. 30% of stabilizer)
Contaminants: Creatinine amidohydrolase ≤1.0×10⁻²%;
Creatine amidinohydrolase ≤1.0×10⁻²%;
Urease ≤1.0×10⁻²%;
NADH oxidase ≤1.0×10⁻²%;
NH4⁺ ≤1.0×10⁻²% µg/u
Stabilizer: Mannitol
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 260,000
Isoelectric point: 4.4
Michaelis constant: 3.5×10⁻³M (Creatinine)
Structure: 6 subunits per enzyme molecule
Inhibitors: Ag⁺, Hg⁺⁺, o-phenanthroline,monoiodoacetate
Optimum pH: 8.5-9.5
Optimum temperature: 65-75℃
pH Stability: pH 7.0-11.0 (30℃, 20hr)
Thermal stability: below 65℃ (pH 7.5, 1hr)
Applications
This enzyme is useful for enzymatic determination of creatinine when coupled with glutamate dehydrogenase in clinical analysis.
Research use only.
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