Stability: (-221) Stable at -20°C for at least One year (-229) Stable at 4°C
Molecular weight: approx. 67,000 (by gel filtration)
Structure: 2 subunits per enzyme molecule
Isoelectric point: 4.5±0.1
Michaelis constant: 4.5×10⁻³ M (Creatine)
Inhibitors: Hg⁺⁺, Cu⁺⁺, Ag⁺, SH reagent (NEM), PCMB
Optimum pH: 6.5-7.5
Optimum temperature: 40-50℃
pH Stability: pH 4.0-10.0 (25℃, 20hr)
Thermal stability: below 50℃ (pH 7.5, 30min)
Applications
This enzyme is useful for enzymatic determination of creatine and creatinine when coupled with creatinine amidohydrolase and sarcosine oxidase in clinical analysis.
