The CopyControl™ Induction Solution is used to induce CopyControl clones developed in the CopyControl pCC1 and pCC2 vectors, grown in TransforMax™ EPI300™ E. coli, from single-copy number to high-copy number.
The Induction Solution induces expression of a mutant trfA gene contained in the TransforMax EPI300 cells. Expression of trfA gene results in initiation of replication from the oriV high-copy origin of replication and subsequent amplification of the CopyControl clones and clones developed in the CopyControl pCC1 and pCC2 vectors.
The CopyControl Induction Solution can be added to CopyControl clones growing in culture or to agar media prior to plating of CopyControl clones transformed into TransforMax EPI300 cells.
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Key features
Control copy of inducible copy-number clones using the CopyControl Cloning System
