Collagenase A is isolated from Clostridium Histolyticum and has the original balance of collagenase proteases. It is widely used for the disaggregation of many types of tissues (e.g., lung, heart, muscle, bone, adipose tissue, liver, kidney, cartilage, mammary gland, placentae, blood vessels, brain, tumors, umbilical cord), and for the preparation of single-cell suspensions for the establishment of primary cell culture systems.
Tribioscience’s Collagenase A is a lyophilized powder, and stable at 2-8°C. It makes it easy to storage and application.
Storage Conditions
Collagenase A is stored at +2 to +8°C for a shelf-life of 1 year. The expiration date is printed on the label.
Reconstitution
Collagenase A is soluble from 1-20 mg/mL with an optimal pH range of 6.3-7.5. It is recommended to reconstitute with a buffer compatible with the intended assay. The recommended working concentration is 0.1-5 mg/mL.
Research use only.
Documents
References
1. Wu, H. F., Saito-Diaz, K., Huang, C. W., McAlpine, J. L., Seo, D. E., Magruder, D. S., Ishan, M., Bergeron, H. C., Delaney, W. H., Santori, F. R., Krishnaswamy, S., Hart, G. W., Chen, Y. W., Hogan, R. J., Liu, H. X., Ivanova, N. B., & Zeltner, N. (2024). Parasympathetic neurons derived from human pluripotent stem cells model human diseases and development. Cell stem cell, 31(5), 734–753.e8. https://doi.org/10.1016/j.stem.2024.03.011
2. Wu, H. F., Ishan, M., Rashid, M. M., Liu, H. X., & Zeltner, N. (2024). Novel Cross-Species Salivary Gland-Parasympathetic Neuron Coculture System. Bio-protocol, 14(21), e5101. https://doi.org/10.21769/BioProtoc.5101
Research use only.
