Preparation and Specification
Appearance: Yellowish amorphous powder, lyophilized
Activity: GradeⅢ 12U/mg-solid or more
Contaminants: Catalase ≤1.0×10-1%, Cholesterol esterase ≤1.0×10-2%
Stabilizers: BSA, amino acids
Properties
Stability: Stable at −20℃ for at least one year
Molecular weight: approx. 55,000 (by gel-filtration)
Isoelectric point: 4.6±0.1, 4.9±0.1 and 5.2±0.1
Michaelis constant: 2.1×10-5M (Cholesterol)
Inhibitors: Ionic detergents, Ag＋, Hg＋＋
Optimum pH: 7.0−8.0
Optimum temperature: 60℃
pH Stability: pH 5.0−10.0 (25℃, 20hr)
Thermal stability: below 55℃ (pH 7.0, 15min)
Applications
This enzyme is useful for enzymatic determination of cholesterol in serum when coupled with cholesterol
esterase in clinical analysis.
Research use only.
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