Tribioscience’s CHO Host Cell Protein ELISA is designed to quantitatively detect CHO HCP levels in samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 1 to 810 ng/mL. The levels of HCP samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for CHO protein.
Typical Data
This standard curve (R2=0.9972) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MDD) of CHO HCP is typically 1 ng/mL.
The Intra-assay CV is ˂ 6.6% and the Inter-assay CV are ˂ 9%.
Specificity
This assay recognizes natural and recombinant CHO HCP.
No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
PART
PART#
DESCRIPTION
STORAGE OF OPENED/ RECONSTITUTED
CHO HCP Microplate
TBS31004A
96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for CHO HCP.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
CHO HCP Standard
TBS31004B
50 µl of Recombinant CHO HCP (8.1 μg/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS31004C
2.1 mL of biotin-CHO HCP antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS31004D
200 µL of streptavidin HRP.
Assay Diluent
TBS31004E
25 mL of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
