The ChIP Elute Kit is designed to be used downstream of a chromatin immunoprecipitation (ChIP) assay to elute DNAassociated with the antibody. The ChIP Elute Kit allows for simultaneous elution and crosslinking reversal of nuclear protein-DNA complexes in a single step. The resulting DNA is single stranded (ssDNA) and is purified and concentrated with the ChIP-grade reagents provided in the kit. The output from this kit is directly compatible with ChIP-seq library preparation using the DNA SMART ChIP-Seq Kit. The DNA SMART ChIP-Seq Kit generates sequencing libraries compatible with Illumina platforms from single-stranded or double-stranded fragmented DNA.
Features
Fast (one hour) cross-link reversal and elution of ChIP DNA
Ligation-independent adapter addition for ChIP-seq library preparation
Flexible DNA inputs: 100 pg–10 ng of either ssDNA or dsDNA
Generate Illumina-ready sequencing libraries in around 4 hours
Applications
ChIP Elute Kit: DNA elution and cross-link reversal for qPCR or ChIP-seq applications DNA SMART ChIP-Seq Kit: Sequencing library preparation for ChIP-seq from low-input, fragmented DNA Illumina-specific NGS sequencing library production
