CFSE, Cellular proliferation probe

Product Details

Formal Name: 5-Carboxyfluorescein N-succinimidyl ester

Alternate Names: 5-Carboxyfluorescein NHS ester; 5-FAM-SE

Molecular Formula: C₂₅H₁₅NO₉

Formula Weight: 473.3

CAS Number: 92557-80-7

Purity: >97%

Formulation: Powder

Solubility: Soluble in DMSO (35 mg/ml)

Storage: -20°C

Stability: ≥ 2 years.

Applications

Cellular proliferation probe

Functions

Fluorophore that can diffuse into cells and covalently react with intracellular proteins via its activated carboxy group and thus be retained in the cell for long periods of time. CFSE fluorescence can be used to analyze cell proliferation, track cell migration and in applications such as flow cytometry. Excitation/emission max: 491 and 518 nm.

Application Procedures

First dissolved in DMSO (35 mg/ml), then diluted to aqueous buffer. Solutions in DMSO may be stored at -20°C for up to 3 months.

Research use only.

Documents

• Datasheet (PDF)