Cellartis 3i mES/iPSC Culture Medium is a serum-free, defined cell culture medium for the establishment, maintenance, and propagation of mouse embryonic stem (mES) cells. This medium contains three selective small molecule inhibitors (3i technology: ERK/MEK inhibitor, GSK3β inhibitor, and FGFR-TK inhibitor) that act to eliminate differentiation-inducing signals and promote cell proliferation, enabling the maintenance of the pluripotent ground state without the the need for additional stimulatory cytokines.
Features
Completely defined, serum-free, feeder-free, and cytokine-free formulation
Uses selective small molecule inhibitors to block differentiation signals and maintain mouse ES cells in a pluripotent state
Helps maintain and propagate mouse ES cells at ≥99% purity
Applications
Maintaining mouse ES cells in a pluripotent state
Deriving mouse ES cell lines from non-permissive mouse strains
Efficiently producing fully ES-cell-derived mice for mouse line establishment
