Cellartis 2i mES/iPSC Culture Medium is a serum-free, defined cell culture medium for the establishment of mouse ES and iPS cell lines. This medium contains two selective small molecule inhibitors ('2i'), CHIR99021 and PD0325901, that act to eliminate differentiation-inducing signals from GSK3β and ERK/MEK, respectively, and promote cell proliferation.
Features
Completely defined, serum-free formulation promotes cell proliferation and maintains pluripotent state
Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK, allowing establishment and long-term maintenance of germline-competent mouse ES and iPS cells
Can be combined with Leukemia Inhibitory Factor (LIF) to induce the efficient conversion of partial- or pre-iPS mouse cells into fully pluripotent iPS cells
Applications
Establishment of mouse ES and iPS cell lines
Long-term maintenance of pure ES or iPS cells
Conversion of partial- or pre-iPS cells into fully pluripotent cells in the presence of LIF
