Perform a simple, two-step real-time RT-PCR, without RNA extraction, directly from animal cells with the CellAmp Direct TB Green RT-qPCR Kit. The kit makes it possible to prepare template samples from cultured cells and complete the analysis of reverse transcription reactions and gene expression in as little as 1.5 hr.
Features
High-throughput analyses can be performed easily and rapidly (~1.5 hr)—no need to do RNA extraction or heat treatment to inactivate DNase, making the process faster and less cumbersome
Can be used with cells differentiated from stem cells and iPS cells—improved lysis buffer works well with a variety of adherent cells, suspension cells, primary cultured cells, various stem cells, iPS cells, etc.
Lysates can be stored for a long time—storage stability has been confirmed at –20°C for at least 6 months
Resistance to PCR inhibitors and high specificity—use of Probe qPCR Mix as the real-time PCR reagent makes this product highly resistant to PCR inhibitors and highly reactive for targets with high GC content
Applications
High-throughput gene expression analyses in a very short time (~1.5 hr) RT-qPCR of stem cells (such as iPS cells, suspension cells, adherent cells, primary cells, etc.) Analysis of samples with high GC content—high specificity and high tolerance to inhibitors Analysis of samples typically impacted b
