The CAR Receptor Booster increases the transduction efficiency of target cells to infection by adenovirus. The CAR Receptor Booster consists of exosome-like particles coated with adenoviral receptor protein CAR. When applied to target cells, fusion of the particles with the host cell plasma membrane temporarily increases surface levels of CAR to allow for efficient adenoviral transduction. Additionally, CAR Receptor Booster renders cells that cannot usually be transduced with adenovirus (e.g. hematopoietic cells) susceptible to infection.
Features
Transduce any cell type with recombinant adenovirus, including hematopoietic cells and CHO cells which are known for having a low density of the CAR receptor
Increase efficiency of transduction by temporarily increasing the concentration of the principal adenoviral receptor proteins on your target cells
Simply preincubate your target cells with these concentrated exosome-like vesicles for 2 hr prior to infection with virus
Increase the cell-surface density of the coxsackie and adenovirus receptor protein (CAR)
Applications
Delivery of adenovirus to cells resistant to infection
