Capturem™ Streptavidin Miniprep Columns

Capturem Streptavidin Miniprep Columns are single-use, disposable columns for simple, rapid enrichment of target proteins and antibodies that bind biotinylated proteins. The membranes are guaranteed to bind more than 15 μg of biotinylated BSA control per column. These miniprep columns are suitable for enrichment of target proteins and antibodies, including those from animal serum, cell culture lysates (e.g., mammalian or bacterial cell lysates), and cell culture supernatants of hybridoma cell lines. Each column can hold up to 850 µl of sample and requires a minimum elution volume of 100 µl.

Features

• Simple, ultra-fast workflow: 5–15-minute room-temperature protocols
• High concentration: extremely small elution volumes yield highly concentrated complexes
• High quality: short residence times reduce the possibility of aggregation, complex dissociation, or loss of activity
• High reproducibility: consistent results from well to well
• Single use: novel membrane technology assembled into disposable spin columns reduces the risk of contamination and carryover

Applications

Purification or enrichment of biotinylated proteins
Pull-down of binding partners (e.g., proteins or oligos) for a biotin-labeled ligand

Documents

• Certificate of Analysis (PDF)
• User Manual (PDF)