The Capturem IP & Co-IP Kit is designed for the simple and rapid capture of protein-protein complexes from whole cell extracts. The kit contains specially designed Protein A spin columns, using our novel Capturem technology for efficient capture of antibody-bound target protein complexes. The included buffer set provides ease of use and rapid isolation of immunoprecipitated protein complexes, allowing faster downstream analysis. Each column can hold up to 850 µl of sample and requires a minimum elution volume of 20 µl.
Features
Simple, ultra-fast workflow: incubate IP antibody with sample for 10 minutes then purify at room temperature in 5 minutes
Complete solution: includes Capturem Protein A spin columns and immunoprecipitation buffers optimized for IP and Co-IP experiments
High concentration: extremely small elution volumes yield highly concentrated complexes
High quality: short residence times reduce the possibility of aggregation, complex dissociation, or loss of activity
Versatility: compatible with a wide range of IP buffers and conditions
Applications
Immunoprecipitation Co-immunoprecipitation Investigation of protein complexes Antigen screening for antibody discovery and engineering workflows
