Capturem His-Tagged Purification Maxiprep Columns are single-use, disposable, membrane spin columns for simple, rapid purification of his-tagged proteins in up to 23 ml of clarified lysate. The columns are suitable for use under native or denaturing conditions and in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol. Each column requires a minimum elution volume of 500 µl.
Features
High purity and yield—small column bed volumes trap fewer contaminants and allow large-volume washes
Compatible with wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, BME, glycerol, TCEP, etc.; see compatibility table)
Lysis buffer compatibility—xTractor Buffer; buffers from other vendors
Different cell systems—easy purification from mammalian and bacterial cells
