Calcium phosphate transfection is a powerful method to introduce DNA plasmid into eukaryotic cells. The principle is to mix phosphate buffer with CaCl2 solution containing DNA plasmid to form DNA-calcium phosphate precipitate. This precipitate can adhere to the cell and eventually transfer DNA into the target cell by endocytosis.
Tribioscience Calcium Phosphate Transfection kit provides high-quality pretested reagents suitable for cell transfection needs. The kit includes all the reagents necessary to perform cell transfection.
Main features
Accurate: Optimized conditions for highly consistent transfections
High efficiency: High transfection efficiency in many types of mammalian cells
Flexible: Multiple size choices for different needs
Kit composition, tests, and storage conditions
Components
TBS8201-40
TBS8201-120
2.5M CaCl2
3.2 mL
9.6 mL
2x HBS
32 mL
96 mL
Sterile Water
32 mL
32 mL
10 cm Plate
40 Tests
120 Tests
6-well Plate
300 Tests
900 Tests
Note: Store the components at 4 °C for a shelf-life of 2 years.
