BspQ I

BspQ I is a type IIS restriction enzyme that creates linearized, scarless templates from plasmid DNA that are suitable for in vitro transcription. BspQ I (10 U/μl) and 10X BspQ I Buffer are included.

Features

• Cuts outside recognition site keeping the poly(A) tail sequence intact, which promotes successful translation downstream of the in vitro transcription
• Linearizes plasmid DNA in one hour
• Comes in an HQ version to support a smooth transition from RUO to GMP grade RNA manufacturing

Applications

Linearization of plasmid DNA for use as a template for in vitro transcription.

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)