BMH-21, RNA polymerase I inhibitor

Product Details

Formal Name: N-[2-(dimethylamino)ethyl]-12-oxo-12H-benzo[g]pyrido[2,1-b]quinazoline-4-carboxamide

Molecular Formula: C₂₁H₂₀N₄O₂

Formula Weight: 360.40

CAS Number: 896705-16-1

Purity: >98%

Formulation: powder

Solubility: Soluble in DMSO (13 mg/ml with warming)

Storage: -20°C

Stability: ≥ 2 years.

Applications

RNA polymerase I inhibitor

Functions

A planar heterocyclic DNA intercalator that inhibits RNA polymerase I (Pol I), IC50 = 0.6 µM in U2OS cells, but not Pols II or III, blocking transcription elongation without inducing the DNA damage response. Inhibition disengages Pol I from chromatin, resulting in proteasome-dependent destruction of Pol I subunit RPA194. At nanomolar concentrations it also activates p53 in A375 cells.

Application Procedures

First dissolved in DMSO (13 mg/ml with warming), then diluted to aqueous buffer. Solutions in DMSO may be stored at -20°C for up to 3 months.

Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)