Isolation of high quality RNA is an important first step in gene expression studies such as RT-PCR, qRT-PCR and array analysis. TBS’s Blood RNA Isolation Kit is designed to isolate total RNA from PBMC. Under acidic conditions of reagent, total RNA remains in the upper aqueous phase, while most of DNA and proteins remain either in the interphase or in the lower organic phase. Total RNA is then recovered by precipitation with isopropanol and can be used for several applications. This kit can significantly improve the quantity and quality of RNA.
Feature :
Simple and convenient: There is no special equipment need.
High yield rate: This method gets higher yield rate of RNA than spin column method.
Versatility: multiple RNAs include microRNA.
Applications :
Total RNA isolation from PBMC. The yield of total RNA from 1 x107 PBMC should range from 50 µg to 80 µg. The A260/A280 ratio of the isolated RNA should be above 1.8.
Storage conditions :
The kit is shipped at room temperature. Store the reagent at 4°C. Shelf life: 12 months after receipt.
