Biotinyl Tyramide, Signal amplification reagent for antibody assays

Product Details

Formal Name: (3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide

Alternate Names: Biotin-phenol

Molecular Formula: C₁₈H₂₅N₃O₃S

Formula Weight: 363.47

CAS Number: 41994-02-9

Purity: >98%

Formulation: powder

Solubility: Soluble in DMSO (up to 35 mg/ml) or in Ethanol (up to 20 mg/ml)

Storage: -20°C

Stability: ≥ 1 year.

Applications

Signal amplification reagent for antibody assays

Functions

Reagent for use in catalyzed reporter deposition (CARD) signal amplification protocols in a variety of immunoassays in which, horseradish peroxidase- catalyzed deposition of biotinyl tyramide is detected with labeled streptavidin. Widely used for signal amplification in fluorescent in situ hybridization (FISH) protocols.  May be used for signal amplification in a sandwich ELISA for quantification of α-tubulin isotypes. Reagent for “self-biotinylation” of DNA G-quadruplexes. May be used with APEX-mediated biotin labeling to identify protein-protein interactions.

Application Procedures

First dissolved in DMSO (up to 35 mg/ml) or in Ethanol (up to 20 mg/ml), then diluted to aqueous buffer. Solutions in

DMSO or ethanol may be stored at -20°C for up to 1 month.

Research use only.

Documents

• Datasheet (PDF)