BcaBEST™ RNA PCR Kit Ver. 1.1

The BcaBEST RNA PCR Kit is designed to perform both reverse transcription and DNA amplification in a single tube. The kit uses BcaBEST DNA Polymerase, which contains both DNA polymerase and reverse transcriptase for first strand cDNA synthesis. In contrast to standard reverse transcriptases, BcaBEST Polymerase has a temperature optimum of 65°C, which permits cDNA synthesis from difficult and highly structured RNA templates. Bca-Optimized Taq Polymerase is used for second strand synthesis and subsequent PCR. This polymerase utilizes LA PCR technology for improved PCR length and accuracy. Random 9-mers, oligo-dT primers, or a specific downstream primer that acts as an antisense primer in PCR can be used for cDNA synthesis.

Features

• Advantage RT-PCR kits
• Library construction kits
• One-step RT-PCR
• PrimeScript Double Strand cDNA Synthesis Kit
• PrimeScript II 1st Strand cDNA Synthesis Kit

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• Components
• Components
• Components