Bacteriophage MS2 RT-PCR Detection Kit is a one-step real-time reverse transcription polymerase chain reaction (RT-PCR) assay intended for the detection of MS2 expressed in bio-samples. It combines both reverse transcription (RT) and Taqman probe-based PCR amplification to occur in a single reaction tube. The kit is optimized for the two reactions in a real-time “single step”. This One-step qRT-PCR assay offers the end-users an efficient, easy-to-use, and reliable alternative to conventional “two-step” sequential qRT-PCR.
Bacteriophage MS2 RT-PCR Detection Kit contains all components for RT-PCR reaction including RT-PCR enzymes, primer-probe, internal control, positive control, negative control, and buffer. The MS2 target gene is labeled fluorescence Fam, and internal control is labeled with Hex.
Applications
This kit is used for the detection of MS2 samples.
Key Features
One-step complete qRT-PCR in a single tube.
Reduce contamination in the operating process.
Accurate detection and quantification of MS2 target gene.
4x RT-PCR mix makes it easier to adjust the sample size in a one-tube reaction.