Bacillus subtilis has become an increasingly popular host for recombinant protein expression. With its ability to secrete protein directly into culture media, amenability to medium- and large-scale fermentation, lack of codon bias, and designation by the U.S. Food and Drug Administration as an organism that is Generally Regarded As Safe (GRAS), it's no wonder that the majority of industrially-produced enzymes are expressed in Bacillus species such as B. subtilis. Optimization of secretion, however, can be necessary to achieve highest yields. To address this, the B. subtilis Secretory Protein Expression System from Takara Bio allows rapid development of a library of B. subtilis clones, each bearing a pBE-S construct in which the ORF for your protein of interest is fused with sequences for 173 unique signal peptides. Perform a downstream assay to identify and select clones which secrete the highest amount of functional protein into the culture media, and you can quickly identify which signal peptide results in the most efficient expression of your desired secreted protein.
Features
Includes pBE-S DNA, an E. coli/B. subtilis shuttle vector containing the B. subtilis-derived subtilisin (aprE) promoter, secretory signal peptide (aprE SP), multiple cloning site (MCS) and 3' (C-terminal) his-tag sequence
Supplied with SP DNA Mixture, a library of DNA sequences encoding 173 unique secretory signal peptides that can be inserted upstream of your target gene
Fully compatible with In-Fusion Cloning kits and systems, allowing rapid and easy construct generation
Includes B. subtilis strain RIK1285
Applications
Optimize secretion by identifying the signal peptide which provides the highest level of expression of secreted protein The clone with the highest level of expression is then ready to use for scaled-up protein expression using B. subtilis
