AutoSeq G-50 columns are specifically designed to remove fluorescent dye-terminators from cycle sequencing reactions prior to analysis on automated sequencers.
For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated DNA sequencing
Columns are prepacked with Sephadex G-50 DNA Grade F
Columns are pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative
Ready-to-use columns enable sample application through to collection of purified product in less than 4 min
4 minutes
Effective DNA purification is essential for high-quality results on automated DNA sequencing since residual dye-terminators can obscure data.
AutoSeq G-50 consists of MicroSpin columns containing Sephadex G-50 DNA Grade F, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artefacts on salt-sensitive automated DNA sequencers.
