Description: The His-tag is an affinity tag widely used to facilitate the purification of recombinant proteins. It typically consists of 6-10 consecutive histidine residues and is introduced at the N- or C-termini of proteins. The tag allows for easy purification via immobilized metal ion affinity chromatography (IMAC) .
Applications:
Elisa
Flow cytometry
Immunoblotting
Immunoprecipitation
Immunocytochemistry
Immunofluorescence
Chromatin immunoprecipitation
Electron microscopy
Supershift assays
Protein purification if conjugated with agarose or magnetic beads
Isotype controls
Molecular Weight: 150 kDa
Host: Mouse
Isotype: Mouse IgG2b kappa
Concentration: 5.0 mg/ml
Purity: >95%
Formulation: PBS, pH 7.0, no stabilizers or preservatives.
Endotoxin: < 1EU/mg
Clonality: Monoclonal
Sterility: 0.2 µm filtered
Storage: 4°C stable for up to one month. Store at -20°C for long-term.
