AmpliSeq for Illumina TCR beta-SR Panel
Vendor: Illumina
SKU: 20031675
In stock
Sufficient for 24 DNA or RNA samples when paired with AmpliSeq Library PLUS for Illumina. Includes AmpliSeq dNTP Mix for Illumina. Purchase library prep, index adapters, and accessory products separately.
AmpliSeq for Illumina TCR beta-SR (Short Read) Panel is a highly multiplexed targeted resequencing panel intended for use with multiple sample types, including formalin-fixed paraffin-embedded (FFPE) tumor samples. It is designed to measure T cell diversity and clonal expansion by sequencing T cell receptor (TCR) beta chain rearrangements. You can use a single pool of multiplex PCR primers, library reagents, and sample barcodes to generate libraries from DNA/RNA extracted from FFPE, whole blood, fresh-frozen tissue, or fluorescence activated cell sorting (FACS)-sorted cells.
Documents
- Automated Solutions for AmpliSeq for Illumina Sequencing Panels
- AmpliSeq for Illumina TCR beta-SR Panel Data Sheet
Support Documentation
Specifications
- Area of interest Cancer research, Cell & molecular biology research, Complex disease genomics, Immuno-oncology research, Immunology
- Assay time 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
- Automation capability Liquid handling robot(s)
- Cancer type Solid tumor
- Content specifications Sequences TCR beta chain rearrangements, with up to 80 bp read-length amplicons for characterizing CDR3.
- Hands on time <1.5 hr
- Input quantity 1 ng
- Instruments MiSeq SystemMiniSeq SystemNextSeq 1000 SystemNextSeq 2000 SystemNextSeq 550 System
- Key use Gene expression analysis, Targeted analysis
- Mechanism of action Multiplex PCR
- Method Amplicon sequencing, Targeted DNA sequencing, Targeted RNA sequencing
- Nucleic acid type DNA, RNA
- Specialized sample types Blood, FFPE tissue
- Species category Human
- Sub category Library preparation kits
- Technology Sequencing
- Variant class Transcript variants
links and documants
- Datasheet url Click here
- Source url Click here
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AmpliSeq for Illumina TCR beta-SR Panel
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