AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel 20024479 - Illumina

AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel

Name: AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel
Brand: Illumina
SKU: 20024479
Availability: InStock

Vendor: Illumina

SKU: 20024479

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In stock

Sufficient for 24 reactions when paired with AmpliSeq Library PLUS for Illumina. Purchase library prep, index adapters, and accessory products separately.

AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel is a highly multiplexed targeted resequencing panel to measure T cell diversity and clonal expansion by sequencing T cell receptor (TCR) beta chain rearrangements. Use a single pool of multiplex PCR primers, library reagents, and sample barcodes, to generate libraries from RNA extracted from whole blood, fresh-frozen tissue, or FACS-sorted cells.

Documents

Support Documentation

Specifications

Area of interest Cancer research, Complex disease genomics, Immuno-oncology research, Immunology
Assay time 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Content specifications RNA evaluation of TCRβ chain rearrangements, including CDR1, CDR2, and CDR3 (with up to 400 bp read-length amplicons)
Hands on time < 1.5 hr
Input quantity 10–1,000 ng
Instruments MiSeq SystemMiniSeq SystemNextSeq 1000 SystemNextSeq 2000 SystemNextSeq 550 System
Key use Targeted analysis
Mechanism of action Multiplex PCR
Method Amplicon sequencing, Targeted RNA sequencing
Multiplexing 96 dual index combinations
Nucleic acid type RNA
Specialized sample types Blood
Species category Human
Sub category Library preparation kits
Technology Sequencing
Variant class Transcript variants

links and documants

Datasheet url Click here
Source url Click here