AmpliSeq for Illumina Myeloid Panel 20024478 - Illumina

Ampliseq cDNA Synthesis for Illumina

Name: Ampliseq cDNA Synthesis for Illumina
Brand: Illumina
SKU: 20022654
Availability: InStock

Vendor: Illumina

SKU: 20022654

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Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels).

The Myeloid Panel enables concurrent analysis of both DNA and RNA from blood and bone marrow samples in a single assay to study biomarkers associated with hematologic malignancies. The panel covers relevant targets for these major myeloid disorders: acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML). Key features include:

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Support Documentation

Specifications

Area of interest Cancer research
Assay time 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Cancer type Hematologic
Content specifications Targets biomarkers across 69 genes (40 key DNA target genes, 29 RNA fusion driver genes, and 5 gene expression levels)
Hands on time < 1.5 hr
Input quantity 20 ng high-quality DNA; 10 ng high-quality RNA (10 ng recommended per pool)
Instruments MiSeq SystemMiniSeq System
Key use Targeted analysis
Method Amplicon sequencing, Targeted DNA sequencing, Targeted RNA sequencing
Multiplexing Up to 96-plex
Nucleic acid type DNA, RNA
Specialized sample types Blood, Bone marrow, Not FFPE-compatible
Species category Human
Sub category Library preparation kits
Technology Sequencing
Variant class Gene fusions, Insertions-deletions (indels), Single nucleotide polymorphisms (SNPs), Somatic variants

links and documants

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