Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.
Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL reagents.
Optimized system provides rapid, sensitive results using simple protocols.
Designed for use with nitrocellulose and PVDF membranes.
High sensitivity: Up to 10 times more sensitive than colorimetric methods.
Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.
Easy reprobing: Save time and materials by reprobing on membranes up to 10 times.
Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,
Non-radioactive-no specialized facilities needed
Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide
Amersham ECL Western Blotting Detection Kit uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.
ECL Western Blotting System is the world’s most widely used and referenced chemiluminescent immunodetection system.
Each system contains the following reagents, sufficient for detection of 1000 cm² membrane:
Anti-mouse HRP conjugate, 100 µl
Anti-rabbit HRP conjugate, 100 µl
ECL Detection Reagents 1 and 2, 62.5 ml each
Blocking agent, 5 g.
