Advanced glycation end products (AGEs) are formed from the nonenzymatic reaction of amino groups with reducing sugars. AGEs have been implicated in diseases, such as diabetes mellitus, non-diabetic nephropathy, macrovascular disease, Alzheimer’s disease, cataract, and aging. AGE receptors, such as the receptor for AGE (RAGE), mediate biological responses to AGEs, including endocytic uptake and degradation and induction of cytokines and growth factors.
Advanced glycation end products of Bovine serum albumin (AGE-BSA) are produced by incubating BSA with glucose, followed by extensive dialysis.
The fluorescence of AGEs was confirmed by fluorescence spectrophotometry with Ex./Em. = 370/440 nm. Glycated BSA shows a 7000% increase in fluorescence compared to control BSA. BSA 10 mg/ml in PBS (Cat. No TBS5074-BSA) can be used as a control for AGE-BSA.
