Advantage® HF 2 PCR Kit

This kit pairs Takara's hot-start Advantage 2 Polymerase Mix with a proprietray dNTP mix to specially amplify fragments up to 3.5 kb with exceptionally high fidelity (30-fold higher than wild-type Taq). Enough reagents are supplied for 100 PCR amplifications of 50 μl each.

Features

• Provides virtually error-free amplification—nearly 30-fold higher fidelity than wild-type Taq
• Produces exceptionally high yields without sacrificing fidelity
• Pre-optimized kit components eliminate the need to optimize reaction conditions
• Generates PCR products (up to 5 kb) that can be cloned into any TA-based vector

Applications

Cloning/TA-cloning
Mutation analysis
Long and accurate PCR
High-fidelity PCR amplification

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)

Kit Components

the hot-start Advantage 2 polymerase blend of Titanium Taq DNA Polymerase and a small amount of proofreading enzyme