The changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Tribioscience’s ADP/ATP Ratio Assay kit utilizes bioluminescent detection of the ADP and ATP levels for rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. ADP level is measured by its conversion to ATP which is subsequently detected using the same reaction. The assay can be fully automatic for high throughput and is more sensitive than other methods used for cell viability assays (can detect 100 mammalian cells/well).
Applications
Cell Proliferation
Cytotoxicity and Apoptosis
HTS
Key Features
Sensitive and Accurate: As low as 100 cells can be accurately quantified.
Simple and High-Throughput: Simple procedure; Can be readily automated on HTS liquid handling systems.
Storage Conditions
Store kit at -20℃. Shelf life of three months. Warm up all components (except enzyme) to room temperature before use. Briefly centrifuge all small vials prior to opening.
