Potent and selective ALK4, 5 and 7 inhibitor. IC50=45, 12 and 7.5 nM respectively. Prevents phosphorylation of Smad2/3 and growth inhibition induced by TGFβ2. Inhibits differentiation of rat induced pluripotent stem cells and increases clonal expansion efficiency. Together with AMI-5, A83-01 enabled Oct4-induced reprogramming of mouse embryonic fibroblasts. Cell permeable. Active in vivo.
Application Procedures
First dissolved in DMSO (up to 20 mg/ml), then diluted to aqueous buffer. Solutions in DMSO may be stored at -20° for up to 2 months.
