5X One Step PrimeScript III RT-qPCR Kit contains a more concentrated version of the premix in our 2X One Step PrimeScript III RT-qPCR Mix (RR600). For example, only 5 μl of the 5X premix is needed for a 25 μl reaction versus 12.5 μl of 2X premix. This opens possibilities for using a more dilute template or multiple primers and probes.
Features
Fast, easy one-step RT-qPCR in a single tube using probe-based detection without the need for any preamplification
Efficient, specific cDNA synthesis and amplification, even from complex RNA templates
Suitable for a wide variety of samples from different sources, including crude RNA extracts
Compatible with automation systems such as the SmartChip Real-Time PCR System
