4-MUG, Beta-galactosidase fluorogenic substrate

Product Details

Formal Name: 4-Methylumbelliferyl β-D-galactopyranoside

Alternate Names: 4-MU-b-D-Gal; MUgal; MUG; MUGA

Molecular Formula: C₁₆H₁₈O₈

Formula Weight: 338.30

CAS Number: 6160-78-7

Purity: >97%

Formulation: powder

Solubility: Soluble in DMSO (up to 40 mg/ml) or in water (up to 1 mg/ml with warming)

Storage: -20°C / protect from light

Stability: ≥ 2 years.

Applications

Beta-galactosidase fluorogenic substrate

Functions

A versatile fluorogenic substrate for β-galactosidase for use in cell extracts or purified enzyme preparations.  May be used as a fluorescent probe for identification of senescent cells based on lysosomal β-galactosidase activity. This substrate produces a water soluble blue fluorescent coumarin fluorophore. Ex: 342 nm; Em: 441 nm

Application Procedures

First dissolved in DMSO (up to 40 mg/ml) or in water (up to 1 mg/ml with warming), then diluted to aqueous buffer. Solutions in DMSO may be stored at -20° for up to 1 month.

Research use only.

Documents

• Datasheet (PDF)