Collection: Prepacked Columns

Pick the column by purification step and scale. Capture: HisTrap (Ni Sepharose) for His-tagged proteins, HiTrap Protein A / MabSelect for antibodies, GSTrap for GST fusions. Intermediate purification: HiTrap and HiScreen ion exchange (Q, SP, Capto) or hydrophobic interaction (Phenyl, Butyl). Polishing and buffer exchange: Superdex and Sephacryl size exclusion in HiLoad or Increase formats, HiTrap Desalting and PD-10 for group separations. 1 mL HiTrap columns run on a syringe, a peristaltic pump or any ÄKTA system; HiScreen and RoboColumn formats are for method scouting and scale-up studies, HiPrep and HiLoad for preparative runs.