{"product_id":"wyatt-microoptilab-refractive-index-detector-for-uhplc","title":"microOptilab Refractive Index Detector for UHPLC","description":"\u003cspan\u003e\u003cp align=\"center\"\u003e\u003cimg alt=\"Optilab refractometer\" class=\"scale-with-grid\" src=\"https:\/\/www.wyatt.com\/wp-content\/uploads\/2019\/10\/microOptilab-Hero-Neon-10-2-19.jpg\" width=\"65%\"\u003e\u003c\/p\u003e\u003c\/span\u003e\n\u003cp\u003eUniversal Detection for UHPLC and SEC-MALS\n\u003c\/p\u003e\u003cp\u003eIndependent of Chromaphores\u003c\/p\u003e\n\u003cp\u003eIndependent of chromaphores or fluorophores, differential Refractive Index (dRI) detectors measure concentrations of all types of samples in almost all solvents. The microOptilab brings the universal detection capabilities, unsurpassed concentration range, and superb sensitivity and linearity – familiar from the \u003ca href=\"https:\/\/www.wyatt.com\/products\/instruments\/optilab-refractive-index-detector.html\"\u003eOptilab\u003csup\u003e™\u003c\/sup\u003e\u003c\/a\u003e – to the domain of UHPLC.\u003c\/p\u003e\n\u003chr class=\"no_line\" style=\"margin: 0 auto 10px auto\"\u003e\n\u003cp\u003eApplication Note\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/wyattfiles.s3.us-west-2.amazonaws.com\/literature\/app-notes\/sec-mals-proteins\/absolute-molar-mass-uhplc.pdf\" target=\"_blank\"\u003e\u003ci class=\"fa fa-file-pdf-o fa-lg\"\u003e\u003c\/i\u003eDownload our Application Note on the microOptilab\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eSEC-MALS with UHPLC\u003c\/p\u003e\n\u003cp\u003eLike standard SEC, UHPLC-SEC analysis relying on column calibration with molecular standards is unreliable when the standards do not accurately reflect your samples in terms of conformation, non-ideal (e.g., hydrophobic) column interactions or reversible association. \u003cb\u003eSEC-MALS\u003c\/b\u003e employs a \u003ca href=\"https:\/\/www.wyatt.com\/products\/instruments\/microDawn-multi-angle-detector-uhplc-sec-mals.html\"\u003emicroDAWN\u003csup\u003e™\u003c\/sup\u003e\u003c\/a\u003e MALS detector downstream of the SEC column and UV detector, to determine molar mass \/ molecular weight distributions of macromolecules independently of column calibration.\u003c\/p\u003e\n\u003cp\u003eRefractive index detection is an important component of any SEC-MALS system, and necessary for analyzing conjugated proteins or polymers devoid of chromaphores. The microOptilab connects with the microDAWN MALS detector for a complete UHP-SEC-MALS setup that transfers the many benefits of SEC-MALS analysis to the realm of UHPLC.\u003c\/p\u003e\n\u003cp\u003eKey benefits\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eVery low band broadening, \u0026lt; 4 µL, to maintain the resolution of very narrow UHPLC peaks\u003c\/li\u003e\n\u003cli\u003eExtraordinary dRI measurement range, -4.7 x 10\u003csup\u003e-3\u003c\/sup\u003e to +4.7 x 10\u003csup\u003e-3\u003c\/sup\u003e RIU (comparable to 25 mg\/mL protein in PBS)\u003c\/li\u003e\n\u003cli\u003eHighly sensitive, ± 1.5 x 10\u003csup\u003e-9\u003c\/sup\u003e RIU across the \u003ci\u003eentire\u003c\/i\u003e measurement range\u003c\/li\u003e\n\u003cli\u003eMeasures solvent refractive index, require for MALS calculations\u003c\/li\u003e\n\u003cli\u003eMeasures at the same wavelength as the MALS detector, for maximum accuracy in MALS calculations\u003c\/li\u003e\n\u003cli\u003eTemperature range 4 °C to 65 °C for proteins and polymers that require a range of temperatures\u003c\/li\u003e\n\u003cli\u003eHigh-resolution, touch-screen front panel displays data graphs and instrument diagnostics, even with the host computer turned off\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eUnparalleled features\u003c\/p\u003e\n\u003cp\u003eConsidering the extremely small peak volumes of UHPLC, band broadening in the microOptilab is kept to the absolute minimum, at less than 4 µL, as compared to 20 - 40 µL or more for standard RI detectors. As a result, the superior resolution from UHPLC columns and instruments will not be compromised with the microOptilab RI detector.\u003c\/p\u003e\n\u003cp\u003eEven though the microOptilab offers a dRI measurement range orders of magnitude beyond that of other UHPLC dRI detectors, unlike other detectors there are no range or gain settings in the microOptilab. The \u003ci\u003efull\u003c\/i\u003e range of instrument detection is always present, and the full sensitivity exists over the \u003ci\u003eentire\u003c\/i\u003e range. The small flow cell and proprietary temperature regulation enable stable RI baselines and signals which further enhance the detector's sensitivity.\u003c\/p\u003e\n\u003cp\u003eUnlike all other dRI detectors, the microOptilab also measures absolute refractive index in order to determine solvent RI, necessary in light scattering calculations and useful in assessing the accuracy of mixing of solvent gradients.\u003c\/p\u003e\n\u003cp\u003eThe microOptilab can be operated \u003ci\u003ebelow\u003c\/i\u003e ambient temperatures as easily as above ambient. No hassle temperature control can be programmed down to 4 °C or as high as 65 °C.\u003c\/p\u003e\n\u003cp\u003eThe microOptilab is compatible with \u003ci\u003eall\u003c\/i\u003e UHPLC systems.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRange (RIU)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e-0.0047 to +0.0047\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePeak-to-peak noise (RIU)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e± 1.5 x 10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eResponse Time\n(User Selectable)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.04 to 5 seconds\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAbsolute Refractive Index Range (RIU)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.2 to 1.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity (RIU)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e± 0.002\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBand Broadening\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 4 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFill Volume (inlet tubing + flow cell)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e13.7 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOutlet tubing (0.030\" ID)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e228 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBack pressure created at inlet port (@ 0.5 mL\/min H2O, 20 °C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;320 psi (22 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum permissible back pressure allowed at outlet port\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e30 psi (2 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eControl Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 °C to 65 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRegulation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e± 0.005 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLight Source\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e660 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSafety Sensors\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eVapor and liquid (leak)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDigital Output\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e25 Hz\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAnalog Output\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e± 10 V or ± 1 V output, 18 bits resolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComputer Interface\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEthernet\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFront Panel Display\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e7\" (178 mm), high-resolution, multi-touch display for viewing\/setting signals, instrument settings and diagnostics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInputs\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAnalog In (16 bits, ± 10 V A\/D), Zero dRI, Purge,\nRecycle In, Autoinject In, Alarm In\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOutputs\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAutoinject Retransmit, Alarm Out, Recycle Out (to switch Orbit between waste and recycle)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDimensions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e58 cm (L) x 36 cm (W) x 18 cm (H)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Wyatt Technology","offers":[{"title":"Standard","offer_id":46488398528591,"sku":"WOP1U","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"658nm","offer_id":46488398561359,"sku":"WOP1U-65","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/microOptilab-Hero-Neon-10-2-19_8df3145a-587e-4105-9a61-39b2759e8c49.jpg?v=1787675377","url":"https:\/\/www.danyel.co.il\/products\/wyatt-microoptilab-refractive-index-detector-for-uhplc","provider":"danyel biotech","version":"1.0","type":"link"}