{"product_id":"wyatt-eclipse-field-flow-fractionation-system","title":"Eclipse Field-Flow Fractionation System","description":"\u003cspan\u003e\u003cp align=\"center\"\u003e\u003cimg alt=\"Eclipse DualTec\" class=\"scale-with-grid\" fetchpriority=\"high\" src=\"https:\/\/www.wyatt.com\/wp-content\/uploads\/2020\/10\/Eclipse-Hero.jpg\" width=\"700\"\u003e\u003c\/p\u003e\u003c\/span\u003e\n\u003cp\u003eThe ultimate separation system for nanoparticles and macromolecules\u003c\/p\u003e\n\u003cp\u003eUniquely Versatile\u003c\/p\u003e\n\u003cp\u003eField-flow fractionation is a uniquely versatile means of size-based separations, capable of fractionating viral particles, liposomes and lipid nanoparticles, emulsions, proteins and aggregates, polysaccharides and technical polymers, engineered nanoparticles, colloidal soil suspensions, chemical mechanical polishing slurries, and many more - with no stationary phase and almost no shear. Separation of biotherapeutics can usually be done in full formulation buffer.\u003c\/p\u003e\n\u003cp\u003eThe Eclipse system is the foundation of FFF-MALS, a powerful technique for separation and comprehensive characterization of macromolecules and nanoparticles from 1 to 1000 nm in size. Eclipse brings FFF to a completely new level, combining exquisite performance and ease of use. Advanced features include the Dilution Control Module (DCM) and the ability to switch seamlessly between FFF and size-exclusion chromatography (SEC) separations. The Mobility add-on system expands Eclipse’s capabilities to include measurements of charge and zeta potential.\u003c\/p\u003e\n\u003chr class=\"no_line\" style=\"margin: 0 auto 0px auto\"\u003e\n\n\u003cdiv class=\"column one-second\" style=\"padding-right:10px\"\u003e\n\u003cp style=\"color:#075c97;\"\u003eSimplicity and quality, with built-in intelligence\u003c\/p\u003e\n\u003cp\u003eThe Eclipse FFF system combines several modules that are integrated seamlessly in VISION\u003csup\u003e™\u003c\/sup\u003e software:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eThe Eclipse FFF flow instrument\u003c\/li\u003e\n\u003cli\u003eWaters\u003csup\u003e™\u003c\/sup\u003e\n Arc\u003csup\u003e™\u003c\/sup\u003e HPLC pump and autosampler\u003c\/li\u003e\n\u003cli\u003eIndustry standard pump and autosampler (legacy only)\u003c\/li\u003e\n\u003cli\u003eEclipse FFF channel\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThese modules may be added:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eIndustry standard fraction collector\u003c\/li\u003e\n\u003cli\u003eMobility EAF4 system\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column one-second\" style=\"padding-right:10px\"\u003e\n\u003cdiv style=\"padding:0px 30px 0px 30px\"\u003e\n\u003cp align=\"center\" style=\"padding-top:80px\"\u003e \u003cimg alt=\"fff-mals\" src=\"https:\/\/www.wyatt.com\/wp-content\/uploads\/2024\/04\/WAT-Arc-HPLC-Optilab-DAWN-Eclipse-1000.jpg\" width=\"80%\"\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003chr class=\"no_line\" style=\"margin: 0 auto 0px auto\"\u003e\n\u003cdiv class=\"column one-second mobile-one\"\u003e\u003cdiv class=\"mcb-column-inner\"\u003e\n\u003cp\u003eEclipse instrument\u003c\/p\u003e\n\u003cp\u003eThe Eclipse FFF instrument is the heart of the FFF system. It incorporates four highly precise and stable flow controllers that ensure robust and repeatable fractionation runs, without the associated pulsation and inherent complexity and low reliability of the multiple pumps found in other FFF systems. \u003c\/p\u003e\n\u003cp\u003eEclipse supports both tip injection and focus-zone injection, enabling use of a variety of FFF separation channels and even a size-exclusion chromatography column.\u003c\/p\u003e\n\u003cp\u003e\u003ci\u003eOptional features:\u003c\/i\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFFF-SEC switching\u003c\/li\u003e\n\u003cli\u003eDilution Control Module (DCM)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e(see Options tab for more information)\u003c\/p\u003e\n\u003cp\u003eThe user interface is based on a 10” multi-touch display that lets users track progress of the method, know about events or conditions that need attention, and perform any necessary manual operations such as flushing the channel. Built-in intelligence provides health indicators and guided user assistance as well as self-diagnostics procedure.\u003c\/p\u003e\n\u003cp\u003eFront end\u003c\/p\u003e\n\u003cp\u003eThe front end for an Eclipse FFF system comprises a Waters and other industry-standard pumps and autosampler. These components are recognized by analytical laboratories as state-of-the-art for reliability and performance:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eLow pump ripple and pulsation\u003c\/li\u003e\n\u003cli\u003eNo wasted sample (no need to overfill the autosampler – 100 µL are loaded for a 100 µL injection)\u003c\/li\u003e\n\u003cli\u003eUsed in tens of thousands of laboratories around the world, including in the demanding bio\/pharmaceutical industry.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003cdiv class=\"column one-second mobile-one\"\u003e\u003cdiv class=\"mcb-column-inner\"\u003e\n\u003cdiv style=\"padding:0px 30px 0px 30px\"\u003e\n\u003cp align=\"center\" style=\"padding-top:30px\"\u003e \u003cimg alt=\"fff-mals\" src=\"https:\/\/www.wyatt.com\/wp-content\/uploads\/2020\/10\/Eclipse-Hero.jpg\" width=\"80%\"\u003e \u003c\/p\u003e\n\u003cp align=\"center\"\u003e \u003cimg alt=\"fff-mals\" src=\"https:\/\/www.wyatt.com\/wp-content\/uploads\/2020\/10\/side-view-eclipse.jpg\" width=\"90%\"\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003chr class=\"no_line\" style=\"margin: 0 auto 0px auto\"\u003e\n\u003cdiv class=\"column one-second mobile-one\"\u003e\u003cdiv class=\"mcb-column-inner\"\u003e\n\u003cp\u003eEclipse FFF channels\u003c\/p\u003e\n\u003cp\u003eFractionation, which is what it’s all about, takes place in the open FFF channel which incorporates a semi-permeable (ultrafiltration) membrane supported by a frit. Eclipse channels offer several key advantages including extra-rigid construction, built-in temperature regulation and built-in dilution control ports. A variety of FFF channels are available to meet different needs – see the Channels tab to learn more.\u003c\/p\u003e\n\u003cp\u003eVISION™\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.wyatt.com\/products\/software\/vision.html\"\u003eVISION software\u003c\/a\u003e is the intelligent human interface to an FFF-MALS system built on Wyatt Technology’s Eclipse™ FFF and DAWN™ MALS instruments. It comprises two primary modules:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eVISION DESIGN™ for in silico method design and optimization\u003c\/li\u003e\n\u003cli\u003eVISION RUN™ for running FFF methods, with continuous system monitoring and self-diagnostics to ensure no wasted runs, lost sample or empty solvent bottle.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eVISION interfaces with ASTRA™ software to coordinate synchronization and real-time display of light scattering data within VISION. Both software packages have been developed under a formal quality system and are available in 21 CFR Part 11 compliant options that fulfill GMP and GLP requirements.\u003c\/p\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003cdiv class=\"column one-second mobile-one\"\u003e\u003cdiv class=\"mcb-column-inner\"\u003e\n\u003cdiv style=\"padding:0px 30px 0px 30px\"\u003e\n\u003cp align=\"center\" style=\"padding-top:30px\"\u003e \u003cimg alt=\"fff-mals\" src=\"https:\/\/www.wyatt.com\/wp-content\/uploads\/2021\/12\/Long-Channel-Exploded-12-21.jpg\" width=\"80%\"\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEclipse\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCompatible with aqueous and organic solvents. Corrosion-resistant from pH 2 to 12. Wetted materials: 316 stainless steel, FFKM perfluoroelastomer, FR3 stainless steel, Vespel. Cross-flow regulation from 6.67 to 0.03 mL\/min.\nPrecision of detector flow rate: 0.1% (with DCM). Dedicated fraction collector output port for minimal peak broadening.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCommunications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEthernet\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInputs\/Outputs\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAlarm Out, Aux In with 18-bit effective resolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSoftware\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eVISION software suite. Comprises VISION DESIGN module for in silico method development and optimization, and VISION RUN for running sequences and coordinating ASTRA data acquisition.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFFF-MALS Solution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBasic FFF-MALS system includes Eclipse instrument, one separation channel (matched to customer application), Waters and other industry-standard pump and autosampler, DAWN MALS instrument, VISION software suite and ASTRA light scattering analysis software.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDilution Control Module\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFactory-installed option. Increases sample concentration at the detector by up to a factor of 10 (up to 5 with negligible loss of resolution), controlled by software.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSEC Switching Option\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFactory-installed option. Allows for automated software-controlled switching between SEC mode and FFF mode.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMobility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAdd-on option. Includes Mobility Module with pH sensor and conductivity sensor with software supported calibration, and Mobility channel. Maximum operating voltage ± 30 V, maximum current 30 mA; constant current or constant voltage mode. Wetted materials: platinum, titanium, polyetherimide, alumina ceramic, PTFE, Viton, PEEK, polycarbonate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetectors and Modules\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePDA, UV\/Vis, FLD, fraction collector, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDLS\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eWyattQELS™ embedded dynamic light scattering module\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSelection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eShort, Long, Dispersion Inlet and Semi-Prep Channels available. Channel types are offered as fixed-height channel (without spacer) and variable-height channel (with spacer) options. All channels are compatible with aqueous and organic solvents and come with DCM port. Maximum pressure 30 bar.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTemperature Regulation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAmbient to 50 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWetted Materials\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e316 stainless steel, fused silica, FFKM perfluoroelastomer, alumina ceramic. Polycarbonate or Mylar according to the selected spacer for variable-height channels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpacers\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSelection ranges from 250 μm to 1000 μm, depending on channel. FFKM perfluoroelastomer-coated polycarbonate for aqueous solvents, Mylar for organic solvents.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMembranes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePre-cut polyethersulfone or regenerated cellulose with molecular-weight cutoff from 2 to 30 kDa. Templates available to cut other materials to size.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDimensions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e58 cm (L) x 36 cm (W) x 26 cm (H) (Eclipse instrument)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.wyatt.com\/products\/eclipse-brochure-request.html\" target=\"_blank\"\u003eBrochure\u003c\/a\u003e\u003c\/p\u003e","brand":"Wyatt Technology","offers":[{"title":"Standard","offer_id":46488398364751,"sku":"WEC","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"with Dilution Control Module","offer_id":46488398397519,"sku":"WECD","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"with Dilution Module \u0026 HP Switch","offer_id":46488398430287,"sku":"WECDSH","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Eclipse-Hero.jpg?v=1787675377","url":"https:\/\/www.danyel.co.il\/products\/wyatt-eclipse-field-flow-fractionation-system","provider":"danyel biotech","version":"1.0","type":"link"}