{"product_id":"wyatt-calypso-ii-composition-gradient-system","title":"Calypso II Composition-Gradient System","description":"\u003cspan\u003e\u003cp align=\"center\"\u003e\u003cimg alt=\"Calypso II\" class=\"scale-with-grid\" src=\"https:\/\/www.wyatt.com\/wp-content\/uploads\/2019\/05\/Calypso.jpg\" title=\"Calypso\" width=\"65%\"\u003e\u003c\/p\u003e\u003c\/span\u003e\n\u003cp\u003eLabel-free, immobilization-free characterization of protein-protein and other macromolecular interactions with composition-gradient multi-angle light scattering.\u003c\/p\u003e\n\u003cp\u003eCG-MALS\u003c\/p\u003e\n\u003cp\u003eThe Calypso II, in conjunction with a \u003ca href=\"https:\/\/www.wyatt.com\/products\/instruments\/dawn-multi-angle-light-scattering-detector.html\"\u003eDAWN\u003csup\u003e™\u003c\/sup\u003e\u003c\/a\u003e or \u003ca href=\"https:\/\/www.wyatt.com\/products\/instruments\/minidawn-multi-angle-light-scattering-detector-sec-mals.html\"\u003eminiDAWN\u003csup\u003e™\u003c\/sup\u003e\u003c\/a\u003e MALS detector, measures binding affinities and absolute, molecular stoichiometries of complex biomolecular interactions.\u003c\/p\u003e\n\u003chr class=\"no_line\" style=\"margin: 0 auto 10px auto\"\u003e\n\u003cp\u003eWeight-Average Molar Mass\u003c\/p\u003e\n\u003cp\u003eIn addition to equilibrium association constants \u003ci\u003eK\u003c\/i\u003e\u003csub\u003ed\u003c\/sub\u003e and absolute stoichiometry of reversible self- and hetero-associations, it can also help determine reaction or aggregation rates, non-specific interaction parameters (virial coefficients) and even automate measurements of weight-average molar mass and \u003ci\u003edn\/dc \u003c\/i\u003efor polymers.\u003c\/p\u003e\n\u003chr class=\"no_line\" style=\"margin: 0 auto 10px auto\"\u003e\n\u003cp\u003eCALYPSO\u003csup\u003e™\u003c\/sup\u003e Software\u003c\/p\u003e\n\u003cp\u003eIncluded with each Calypso is a copy of \u003ca href=\"https:\/\/www.wyatt.com\/products\/software\/calypso-software.html\"\u003eCALYPSO software\u003c\/a\u003e, the most versatile software package available for analysis of biomolecular interactions in solution by light scattering.\u003c\/p\u003e\n\u003cdiv class=\"column one-second\"\u003e\n\u003cp\u003eCreating composition gradients...\u003c\/p\u003e\n\u003cp\u003eCG-MALS employs a Calypso connected to a MALS instrument and optional concentration detector. The Calypso performs sample preparation and delivery, combining up to three different solution in precise mixing ratios and injecting into the detectors.\u003c\/p\u003e\n\u003cp\u003eAccurate compositions result from setting the Calypso's pumps to appropriate flow rate ratios, all controlled from the CALYPSO\u003csup\u003e™\u003c\/sup\u003e software through an intuitive GUI. Mixing is achieved by dispensing the solutions simultaneously from all three pumps via a static mixer.\u003c\/p\u003e\n\u003cp\u003e...that work with MALS detectors\u003c\/p\u003e\n\u003cp\u003eReliable light scattering measurement require the samples to be degassed and filtered. The Calypso incorporates built-in, low-volume degassers and filter housings, connected via PEEK tubing and finger-tight fittings. Even the filter housing is finger-tight, making use of a proprietary design for maximum convenience.\u003c\/p\u003e\n\u003cp\u003eCalypso features\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eBiocompatible wetted materials.\u003c\/li\u003e\n\u003cli\u003eFlexibility to re-organize fluid paths and utilize different wetted materials as needed.\u003c\/li\u003e\n\u003cli\u003eConvenient loading of sample and diluent solutions via conical tubes: 5, 15 or 50 mL.\u003c\/li\u003e\n\u003cli\u003eAutoinject Out contact closure signal, to trigger data acquisition by \u003ca href=\"https:\/\/www.wyatt.com\/products\/software\/astra.html\"\u003eASTRA\u003csup\u003e™\u003c\/sup\u003e\u003c\/a\u003e or \u003ca href=\"https:\/\/www.wyatt.com\/products\/software\/dynamics.html\"\u003eDYNAMICS\u003csup\u003e™\u003c\/sup\u003e\u003c\/a\u003e software for data acquisition and analyses not supported by the CALYPSO software (e.g., DLS acquisition).\u003c\/li\u003e\n\u003cli\u003eBuilt-in Wash port, allowing the pumps to draw on reservoirs of wash solutions for post-experimental clean-up. Automation of up to two sequential wash solutions, e.g. buffer (to clean out proteins) followed by water (to clean out buffer salts) or detergent followed by water, is possible when an \u003ca href=\"https:\/\/store.wyatt.com\/shop\/eclipse\/dualtec-o\/orbit\/\"\u003eOrbit\u003csup\u003e™\u003c\/sup\u003e solvent selector valve\u003c\/a\u003e is added to the setup. These operations are appended to the Method for unattended operation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column one-second\"\u003e\n\u003c!--[video_embed video=\"\" parameters=\"\" mp4=\"https:\/\/www.labtube.tv\/videos\/720p\/151907-720.mp4\" ogv=\"\" placeholder=\"\" width=\"400\" height=\"215\"]  \t\n\n          \u003cp\u003e\u003cb\u003ePlay video to learn more about the Calypso system\u003c\/b\u003e\u003c\/p\u003e\n  \t\t\u003cp\u003e\n           \n      \u003c\/p\u003e\t--\u003e\n\u003cimg align=\"right\" alt=\"Calypso Experimental Setup\" height=\"399px\" src=\"https:\/\/www.wyatt.com\/wp-content\/uploads\/2019\/05\/Calypso-DAWN-Optilab-Neon-System.jpg\" style=\"margin: 15px\" width=\"400px\"\u003e\n\u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSyringe Pumps\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3 computer-controlled pumps; dc-servo motor, for minimum pulsation; 3-port distribution valve on each pump for Input, Output and Wash\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSyringe Volumes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e12.5 µL to 2.5 mL (1 mL and 0.5 mL syringes supplied as standard)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDegasser Channels\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 per pump, 60 µL internal volume\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePressure Limit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 mL syringes: 200 psi 0.5 mL syringes: 400 psi\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAuxiliary Inputs\/Outputs\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAutoinject out, injector valve control, recycle valve control, digital and analog I\/O for specific applications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSolvents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAqueous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWetted Materials\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBorosilicate glass, PEEK, PTFE, polyethylene, alumina, titanium, stainless steel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTypical Sample Volume Per Analysis\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2.5 to 7 mL, depending on detector configuration and complexity of analysis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRange of Measurement\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEquilibrium Dissociation Constant, K D : 100 pM to 1 mM typical for 100 kDa molecules (actual range varies with molecular weight and association stoichiometry)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRepeatability of Measurement\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eM w : ± 5% A 2 : ± 0.5 x 10 mol·mL\/g² for sample molar mass of 50 to 100 kDa log( K D ): ± 0.3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecific Binding\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSee CALYPSO Software for more details. Self-association Hetero-association Multi-valent Combined self- and hetero-association incompetent (inactive) fraction Cooperativity and competitive binding Binding with thermodynamic non-ideality (high concentration) Concatenate multiple data sets for global fit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eNon-Specific Interactions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSelf-interaction: 2 and 3 virial coefficients Hetero-interactions: cross virial coefficients with 2 self-virial coefficients\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eKinetics\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 -order analyzed in software; export data for higher-order kinetics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAdditional analyses\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003edn\/dc\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eHost PC Requirements\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUSB port for Calypso Ethernet connection for DAWN \/ miniDAWN communications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDimensions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e58 cm (L) x 37 cm (W) x 35 cm (H)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.wyatt.com\/products\/calypso-brochure-request.html\" target=\"_blank\"\u003eBrochure\u003c\/a\u003e\u003c\/p\u003e","brand":"Wyatt Technology","offers":[{"title":"Default Title","offer_id":46488399020111,"sku":"WCG3","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Calypso.jpg?v=1787675387","url":"https:\/\/www.danyel.co.il\/products\/wyatt-calypso-ii-composition-gradient-system","provider":"danyel biotech","version":"1.0","type":"link"}