{"product_id":"vh3-ligand-elisa-kit-29737000","title":"VH3 ligand ELISA kit","description":"\u003cp\u003eThe VH3 ligand ELISA kit contains all the reagents required to perform the assay, including the VH3 ligand optimized for use in ELISA. The VH3 ligand ELISA kit was developed with a focus on usability, robustness, and environmental sustainability. VH3 ligand ELISA kits are specifically designed for use with MabSelect™ VH3 chromatography resins .\u003c\/p\u003e\n\u003cp class=\"-padding--title-md\"\u003eAccelerate process development with matched ligand leakage ELISA kits\u003c\/p\u003e\n\n\u003cp class=\"-padding--title-md\"\u003eHigh drug product tolerance\u003c\/p\u003e\n\n\u003cp\u003eDownstream samples generally have high concentrations of drug substance. This creates a challenge for protein A measurement. Accurate measurement requires dissociation of the ligand and drug substance, which is especially tricky when the concentration of protein A ligand is very low (\u0026lt; 1 ppm) due to the large excess of drug substance.\u003c\/p\u003e\n\n\u003cp\u003eTo determine the effect of IgG on dissociation, we spiked high, medium, and low concentrations of VH3 ligand into decreasing concentrations of IgG. The VH3 ligand ELISA kit could accurately determine VH3 concentration in samples containing as high as 30 mg\/mL of IgG in the sample.\u003c\/p\u003e\n\n\u003cp class=\"-padding--title-md\"\u003eAvoid unexpected leached VH3 ligand levels in later development\u003c\/p\u003e\n\n\u003cp\u003eThe VH3 ligand ELISA kit is capable of detecting low amounts of ligand, including at the final stages of purification. Leached protein A concentration in downstream materials is usually very low. Therefore, a highly sensitive assay is required to measure leached ligand in these types of samples. The VH3 ligand ELISA kit has a lower limit of detection (LLD) of 10 pg\/mL and a lower limit of quantification (LLQ) of 10 pg\/mL.\u003c\/p\u003e\n\n\u003cp class=\"-padding--title-md\"\u003eA robust and reproducible assay\u003c\/p\u003e\n\n\u003cp\u003eIt is important to have an assay that can give accurate and reproducible results over multiple experiments. Assay precision is the coefficient of variation (CV) within a single assay (intra-assay) and across several experiments (inter-assay).\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eIntra-assay variation\u003c\/strong\u003e was assessed from the interpolated concentration of 155 in-process sample measurements. 89% of samples had an intra-assay CV \u0026lt;5%, with a mean CV of 2.10%.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eInter-assay precision\u003c\/strong\u003e was assessed from spiked samples containing 2500, 1000, 500, or 50 pg\/mL VH3, with and without IgG. The average recovery of these samples was 96.80%, with a mean CV of 8.28%.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/ANy4PIAPQciMijGNFb3J2Q-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29741900.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"96 well plate \/ 1 unit","offer_id":45982290542671,"sku":"29737000","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/xJCk6TFNRe-SLfZ36e64nA-original-png_191bf674-688f-4ff8-ade5-6b5700efe443.png?v=1785959977","url":"https:\/\/www.danyel.co.il\/products\/vh3-ligand-elisa-kit-29737000","provider":"danyel biotech","version":"1.0","type":"link"}