{"product_id":"vero-cell-host-cell-protein-elisa-tbs31005","title":"Vero Cell Host Cell Protein ELISA","description":"\u003cp\u003eThe Tribioscience Vero cell HCP Fast ELISA is designed to quantitatively detect Vero cell HCP residues in different biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay flexible, accurate, simple, and time saving. The measurement can be finished in 2 hours, not needing 4-5 hours (Fig. 1). The detection range is from 1 to 200 ng\/mL. The levels of Vero HCP samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for Vero host cell protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eLOD is about 1.1 ng\/mL\u003c\/p\u003e\n\u003cp\u003eLOQ is 4.9 ng\/mL\u003c\/p\u003e\n\u003cp\u003eThe Intra-assay CV is 3.79% and the Inter-assay CV is ˂10%.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes Vero HCP.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Procedures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-37461 size-full\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/04\/TBS31005-01.jpg\" alt=\"\" width=\"437\" height=\"130\" data-lazy-sizes=\"auto, (max-width: 437px) 100vw, 437px\"\u003e\u003c\/p\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\u003cimg class=\"alignnone wp-image-37463 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/04\/TBS31005_02.png\" alt=\"\" width=\"660\" height=\"350\" data-lazy-sizes=\"auto, (max-width: 660px) 100vw, 660px\"\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-37462 \" style=\"font-size: 16px;font-weight: 400\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/04\/TBS31005_03.png\" alt=\"\" width=\"407\" height=\"296\" data-lazy-sizes=\"auto, (max-width: 407px) 100vw, 407px\"\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%\" border=\"1px solid black\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"83\"\u003e\u003cstrong\u003ePART\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"61\"\u003e\u003cstrong\u003ePART#\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"256\"\u003e\u003cstrong\u003eDESCRIPTION\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"277\"\u003e\n\u003cstrong\u003eSTORAGE OF \u003c\/strong\u003e\u003cstrong\u003eOPENED\/ \u003c\/strong\u003e\u003cstrong\u003eRECONSTITUTED\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"83\"\u003eVero HCP Microplate\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS31005A\u003c\/td\u003e\n\u003ctd width=\"256\"\u003e96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific to Vero HCP.\u003c\/td\u003e\n\u003ctd width=\"277\"\u003eReturn unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"83\"\u003eVero HCP Standard\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS31005B\u003c\/td\u003e\n\u003ctd width=\"256\"\u003e90 µL of Vero HCP protein (2 µg\/mL).\u003c\/td\u003e\n\u003ctd width=\"277\"\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"83\"\u003eDetection A\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS31005C\u003c\/td\u003e\n\u003ctd width=\"256\"\u003e55 µL of HRP Vero HCP antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"5\" width=\"277\"\u003e \n\u003cp\u003eMay be stored for up to\u003c\/p\u003e\n\u003cp\u003e3 months at 2-8 °C.*\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"83\"\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS31005D\u003c\/td\u003e\n\u003ctd width=\"256\"\u003e25 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"83\"\u003eWash Buffer\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3000W\u003c\/td\u003e\n\u003ctd width=\"256\"\u003e15 mL of concentrated solution (10x)\u003cem\u003e.\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"83\"\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3000T\u003c\/td\u003e\n\u003ctd width=\"256\"\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"83\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd width=\"61\"\u003eTBS3000S\u003c\/td\u003e\n\u003ctd width=\"256\"\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration date.\u003c\/p\u003e\n\u003cp\u003eThe kit contains sufficient materials to run an ELISA on one 96 well plate.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986373828687,"sku":"TBS31005","price":3099.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS31005.png?v=1782809760","url":"https:\/\/www.danyel.co.il\/products\/vero-cell-host-cell-protein-elisa-tbs31005","provider":"danyel biotech","version":"1.0","type":"link"}