{"product_id":"ultra-sensitive-human-il-33-elisa-tbs3245","title":"Ultra-sensitive Human IL-33 ELISA","description":"\u003cp\u003eThe Tribio® Ultra-sensitive Human IL-33 Fast ELISA is a solid phase ELISA designed to measure human IL-33 levels in cell culture supernatants, serum, and plasma. \u003cspan style=\"color: #3366ff\"\u003eThe main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and ultra-sensitive. The measurement can detect a concentration as low as 0.5pg\/mL, and finish in 2 hours, with no need for 5-6 hours (Fig. 1). The detection range is from 0.5 to 2048 pg\/mL.\u003c\/span\u003e The levels of human IL-33 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human IL-33 protein.\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Procedures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-7852 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/07\/TBS3245-0.png\" alt=\"\" width=\"625\" height=\"183\" data-lazy-sizes=\"auto, (max-width: 625px) 100vw, 625px\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-11194 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/07\/TBS3245-3.jpg\" alt=\"\" width=\"738\" height=\"358\" data-lazy-sizes=\"auto, (max-width: 738px) 100vw, 738px\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-11195 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/07\/TBS3245-2.png\" alt=\"\" width=\"364\" height=\"326\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 289px\" border=\"1px solid black\" width=\"1123\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-33 Microplate\u003c\/td\u003e\n\u003ctd\u003eTBS3245A\u003c\/td\u003e\n\u003ctd\u003e96 well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IL-33.\u003c\/td\u003e\n\u003ctd\u003eReturn unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-33 Standard\u003c\/td\u003e\n\u003ctd\u003eTBS3245B\u003c\/td\u003e\n\u003ctd\u003e100 µL of Recombinant human IL-33 protein (100 ng\/mL).\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS3245C\u003c\/td\u003e\n\u003ctd\u003e2.2 ml of HRP-Human IL-33 antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS3245D\u003c\/td\u003e\n\u003ctd\u003e12 ml of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS3000T\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003eTBS3000S\u003c\/td\u003e\n\u003ctd\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003cbr\u003e\nThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eRelated Products\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-1-s-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-1ß ELISA (TBS3219)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-2-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-2 ELISA (TBS3220)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-4-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-4 ELISA (TBS3221)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-6-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-6 ELISA (TBS3223)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-7-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-7 ELISA (TBS3224)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS3245--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/07\/TBS3245_human_IL33_elisa_V02_2021.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/07\/TBS3245_Human-IL-33_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReferences\u003c\/h2\u003e\n\u003cp\u003e1. Tan, M., Ye, W., Liu, Y., Chen, X., Huttad, L., Chua, M. S., \u0026amp; So, S. (2024). Niclosamide Prodrug Enhances Oral Bioavailability and Targets Vasorin-TGFβ Signaling in Hepatocellular Carcinoma. \u003cem\u003ebiorxiv\u003c\/em\u003e. \u003ca href=\"https:\/\/doi.org\/10.1101\/2024.10.15.618538\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.1101\/2024.10.15.618538\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e2. Tan, M., Ye, W., Liu, Y., Chen, X., Huttad, L., Chua, M. S., \u0026amp; So, S. (2025). Niclosamide Prodrug Enhances Oral Bioavailability and Efficacy Against Hepatocellular Carcinoma by Targeting Vasorin-TGFβ Signaling. \u003cem\u003eAuthorea\u003c\/em\u003e. \u003ca href=\"https:\/\/doi.org\/10.22541\/au.174119415.55528730\/v1\" target=\"_blank\" rel=\"nofollow noopener\"\u003ehttps:\/\/doi.org\/10.22541\/au.174119415.55528730\/v1\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986382676047,"sku":"TBS3245","price":2050.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3245_IL33.jpg?v=1782810029","url":"https:\/\/www.danyel.co.il\/products\/ultra-sensitive-human-il-33-elisa-tbs3245","provider":"danyel biotech","version":"1.0","type":"link"}