{"product_id":"tribo-tryptase-activity-assay-tbs2101","title":"Tribo™ Tryptase Activity Assay","description":"\u003cdiv class=\"wpb-content-wrapper\"\u003e\n\u003cdiv class=\"vc_row wpb_row row top-row\"\u003e\u003cdiv class=\"vc_column_container col-md-12\"\u003e\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\t\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\t\t\u003cdiv class=\"wpb_wrapper\"\u003e\n\t\t\t\u003cp\u003eTryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules, comprising up to 20% of the total protein of mast cells derived from lung, colon, and skin tissue. Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases.\u003c\/p\u003e\n\u003cp\u003eThe Tribio\u003cstrong\u003e\u003csup\u003eTM\u003c\/sup\u003e\u003c\/strong\u003e Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for evaluation of tryptase activity in cell lysates, supernatants or for inhibitor screening.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eApplications\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCell culture supernatants, cell lysates, or other tryptase-containing supplies.\u003c\/li\u003e\n\u003cli\u003eTesting of purified tryptase enzyme, \u003ci\u003ein vitro\u003c\/i\u003e inhibitor screening.\u003c\/li\u003e\n\u003cli\u003eCell degranulation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKey Features\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitive and Accurate:\u003c\/strong\u003e The kit detects as low as 10μM in solution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSimple and High-Throughput:\u003c\/strong\u003e The procedure is easily adapted to automation with no separation required as a high-throughput assay.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS2101--\u003e\u003c\/p\u003e\n\n\t\t\u003c\/div\u003e\n\t\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/div\u003e\u003c\/div\u003e\n\u003cdiv class=\"vc_row wpb_row row top-row\"\u003e\n\u003cdiv class=\"vc_column_container col-md-8\"\u003e\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\t\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\t\t\u003cdiv class=\"wpb_wrapper\"\u003e\n\t\t\t\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Contents (100 assay)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 60%\" border=\"1px solid black\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"155\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"167\"\u003e\u003cstrong\u003ePart Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"155\"\u003e5x Assay Buffer\u003c\/td\u003e\n\u003ctd width=\"167\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"155\"\u003eTryptase inhibitor\u003c\/td\u003e\n\u003ctd width=\"167\"\u003e200 µL (10mM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"155\"\u003eTryptase Substrate\u003c\/td\u003e\n\u003ctd width=\"167\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"155\"\u003epNA Standard\u003c\/td\u003e\n\u003ctd width=\"167\"\u003e100 µL (10 mM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"155\"\u003eCalcium Ionophore\u003c\/td\u003e\n\u003ctd width=\"167\"\u003e50 µL (1mM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Positive Control is not included in the kit. We recommend that our tryptase recombinant α\/β-1 protein enzyme (catalog: TBP0099). This enzyme was validated with this kit.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\n\t\t\u003c\/div\u003e\n\t\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003cdiv class=\"vc_column_container col-md-4 vc_custom_1758229947708\"\u003e\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\u003cdiv class=\"woocommerce \"\u003e\n\u003cul class=\"products products-container grid pcols-lg-4 pcols-md-3 pcols-xs-3 pcols-ls-2 pwidth-lg-4 pwidth-md-3 pwidth-xs-2 pwidth-ls-1 is-shortcode\" data-product_layout=\"product-outimage\"\u003e\n\n\u003cli class=\"product-col product-outimage product type-product post-25991 status-publish first instock product_cat-protein-and-peptide has-post-thumbnail taxable shipping-taxable purchasable product-type-variable\"\u003e\n\u003cdiv class=\"product-inner\"\u003e\n\t\n\t\u003cdiv class=\"product-image\"\u003e\n\n\t\t\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/protein-and-peptide\/recombinant-human-tryptase-alpha-beta-1-protein-enzyme-activity-tbp0099\/\" aria-label=\"product\"\u003e\n\t\t\t\u003cdiv class=\"inner\"\u003e\u003cimg width=\"300\" height=\"338\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0099-5UG-300x338.jpg\" class=\"wp-post-image lazyloaded\" alt=\"\" data-was-processed=\"true\"\u003e\u003c\/div\u003e\t\t\u003c\/a\u003e\n\t\t\t\u003c\/div\u003e\n\n\t\u003cdiv class=\"product-content\"\u003e\n\t\t\u003cspan class=\"category-list\"\u003e\u003ca href=\"https:\/\/tribioscience.com\/product-category\/life-sciences\/protein-and-peptide\/\" rel=\"tag\"\u003eProtein and Peptide\u003c\/a\u003e\u003c\/span\u003e\n\t\t\t\u003ca class=\"product-loop-title\" href=\"https:\/\/tribioscience.com\/product\/life-sciences\/protein-and-peptide\/recombinant-human-tryptase-alpha-beta-1-protein-enzyme-activity-tbp0099\/\"\u003e\n\t\u003cp\u003eRecombinant human Tryptase alpha\/beta-1 protein, Enzyme Activity (TBP0099)\u003c\/p\u003e\t\u003c\/a\u003e\n\t\n\t\t\n\n\u003cdiv class=\"rating-wrap\"\u003e\n\t\u003cdiv class=\"rating-content\"\u003e\u003cdiv class=\"star-rating\" title=\"\" data-bs-original-title=\"0\"\u003e\u003cspan style=\"width:0%\"\u003e\u003cstrong class=\"rating\"\u003e0\u003c\/strong\u003e out of 5\u003c\/span\u003e\u003c\/div\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n\t\u003cspan class=\"price\"\u003e\u003cspan class=\"woocommerce-Price-amount amount\"\u003e\u003cbdi\u003e\u003cspan class=\"woocommerce-Price-currencySymbol\"\u003e$\u003c\/span\u003e210.00\u003c\/bdi\u003e\u003c\/span\u003e – \u003cspan class=\"woocommerce-Price-amount amount\"\u003e\u003cbdi\u003e\u003cspan class=\"woocommerce-Price-currencySymbol\"\u003e$\u003c\/span\u003e640.00\u003c\/bdi\u003e\u003c\/span\u003e\u003c\/span\u003e\n\n\t\t\u003cdiv class=\"add-links-wrap\"\u003e\n\t\u003cdiv class=\"add-links clearfix\"\u003e\n\t\t\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/protein-and-peptide\/recombinant-human-tryptase-alpha-beta-1-protein-enzyme-activity-tbp0099\/\" data-quantity=\"1\" class=\"viewcart-style-2 button product_type_variable add_to_cart_button\" data-product_id=\"25991\" data-product_sku=\"TBP0099\" aria-label=\"Select options for “Recombinant human Tryptase alpha\/beta-1 protein, Enzyme Activity (TBP0099)”\" rel=\"nofollow\"\u003eSelect options\u003c\/a\u003e\u003cdiv class=\"quickview\" data-id=\"25991\" title=\"\" data-bs-original-title=\"Quick View\"\u003eQuick View\u003c\/div\u003e\t\u003c\/div\u003e\n\t\u003c\/div\u003e\n\t\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\u003c\/div\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"vc_row wpb_row row top-row\"\u003e\u003cdiv class=\"vc_column_container col-md-12\"\u003e\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\t\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\t\t\u003cdiv class=\"wpb_wrapper\"\u003e\n\t\t\t\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eStorage Conditions:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore the kit at -20℃, protect from light.\u003cbr\u003e\nShelf life: 12 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eStandard Preparation (Fig.1)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cimg class=\"alignnone wp-image-34280 size-full\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/02\/TBS2101_01.png\" alt=\"\" width=\"620\" height=\"424\" data-lazy-sizes=\"auto, (max-width: 620px) 100vw, 620px\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eFig. 2: pNA Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cimg class=\"alignnone wp-image-10096 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/02\/TBS2101-3-e1758154298777.png\" alt=\"\" width=\"458\" height=\"334\" data-lazy-sizes=\"auto, (max-width: 458px) 100vw, 458px\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eFig. 3: Tryptase Positive Control Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-10095 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/02\/TBS2101-4.png\" alt=\"\" width=\"425\" height=\"251\" data-lazy-sizes=\"auto, (max-width: 425px) 100vw, 425px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eFig. 4: Effects of pH Value on Tryptase Activity \u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cimg class=\"alignnone wp-image-10094 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/02\/TBS2101-5.png\" alt=\"\" width=\"406\" height=\"403\" data-lazy-sizes=\"auto, (max-width: 406px) 100vw, 406px\" data-was-processed=\"true\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAlternative names: Mast Cell Tryptase, Tryptase enzyme\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\n\t\t\u003c\/div\u003e\n\t\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/div\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/02\/TBS2101_tryptase_assay_v06_2025.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/02\/TBS2101_Tryptase-Assay_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReferences\u003c\/h2\u003e\n\u003cp\u003eYasir, M., Park, J., Han, E. T., Park, W. S., Han, J. H., \u0026amp; Chun, W. (2024). Identification of Potential Tryptase Inhibitors from FDA-Approved Drugs Using Machine Learning, Molecular Docking, and Experimental Validation. ACS omega, 9(37), 38820–38831. \u003ca href=\"https:\/\/doi.org\/10.1021\/acsomega.4c04886\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.1021\/acsomega.4c04886\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986451193935,"sku":"TBS2101","price":1276.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2101.jpg?v=1782811258","url":"https:\/\/www.danyel.co.il\/products\/tribo-tryptase-activity-assay-tbs2101","provider":"danyel biotech","version":"1.0","type":"link"}