{"product_id":"tribo-reverse-transcription-reaction-kit-tbs4006","title":"Tribo™ Reverse Transcription Reaction Kit","description":"\u003cp\u003eTribo™ Reverse Transcription Kit is optimized to synthesize first-strand cDNA for PCR or qPCR. The optimal first-strand cDNA synthesis temperature is 50°C. The reverse transcription reaction at higher temperatures improves cDNA yields for difficult RNA transcription. This pre-mixed 5X concentrated solution includes all necessary components for first-strand synthesis. With the mixture of 5X RT buffer, dNTPs, MgCl\u003csub\u003e2\u003c\/sub\u003e, DTT, primers (optimized mixture oligo (dT) and random primers), RNase inhibitors, and Reverse Transcriptase, the first-strand cDNA synthesis process is greatly simplified.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eApplications:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit can be used for first-strand cDNA synthesis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKey Features:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul style=\"list-style-type: disc\"\u003e\n\u003cli\u003eEasy-to-use pre-mixed package (All in One)\u003c\/li\u003e\n\u003cli\u003eHigh fidelity, yield, and sensitivity\u003c\/li\u003e\n\u003cli\u003eBroader linear range\u003c\/li\u003e\n\u003cli\u003eComplete cDNA synthesis guaranteed\u003c\/li\u003e\n\u003cli\u003eRobust and high throughput\u003c\/li\u003e\n\u003cli\u003eWork with difficult sequences\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKit Contents:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e5x RT reagent: 0.2mL\u003cbr\u003e\nThe kit contains RT buffer, dNTPs, DTT, RNase inhibitor, Primer mixer, and Reverse Transcriptase. The reagent is enough for 50 RT reactions in 20μL.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eStorage Conditions:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe kit is shipped on ice. All components should be stored at -20°C in a non-frost-free freezer.\u003cbr\u003e\nShelf life is 12 months after receiving.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS4006--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/01\/TBS4006_Reverse-Transcription-Reaction-Kit_V03_2020.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/01\/TBS4006_Reverse_Transcription_Reaction_Kit_MSDS-2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReferences\u003c\/h2\u003e\n\u003cp\u003e1. Ramos-León, J., Valencia, C., Gutiérrez-Mariscal, M., Rivera-Miranda, D.-D.-A., García-Meléndrez, C., \u0026amp; Covarrubias, L. (2023, March 09). The loss of antioxidant activities impairs intestinal epithelium homeostasis by altering lipid metabolism. bioRxiv. \u003ca href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2023.03.09.531979v1\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/www.biorxiv.org\/content\/10.1101\/2023.03.09.531979v1\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e2. Ramos-León, J., Valencia, C., Gutiérrez-Mariscal, M., Rivera-Miranda, D. A., García-Meléndrez, C., \u0026amp; Covarrubias, L. (2024). The loss of antioxidant activities impairs intestinal epithelium homeostasis by altering lipid metabolism. \u003cem\u003eExperimental cell research\u003c\/em\u003e, \u003cem\u003e437\u003c\/em\u003e(1), 113965. \u003ca href=\"https:\/\/doi.org\/10.1016\/j.yexcr.2024.113965\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.1016\/j.yexcr.2024.113965\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e3. Arjavalingam, A. (2022). Modeling Neuronal Circadian Rhythms in Major Depressive Disorder using Human Induced Pluripotent Stem Cells. \u003cem\u003eUC San Diego. \u003c\/em\u003e \u003ca href=\"https:\/\/escholarship.org\/uc\/item\/9kf1j00p\" target=\"_blank\" rel=\"nofollow noopener\"\u003ehttps:\/\/escholarship.org\/uc\/item\/9kf1j00p\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986391883855,"sku":"TBS4006","price":583.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS4006.jpg?v=1782810498","url":"https:\/\/www.danyel.co.il\/products\/tribo-reverse-transcription-reaction-kit-tbs4006","provider":"danyel biotech","version":"1.0","type":"link"}