{"product_id":"tribo-mouse-tnf-elisa-kit-tbs3050-01","title":"Tribo™ Mouse TNF-α ELISA Kit","description":"\u003cp\u003eTribioscience’s Fast Mouse TNF-α ELISA is designed to quantitatively detect mouse TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 31 to 2000 pg\/mL. The levels of mouse TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse TNF-α protein.\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve (R\u003csup\u003e2\u003c\/sup\u003e=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of mouse TNF-α is typically 10 pg\/ml.\u003c\/p\u003e\n\u003cp\u003eThe Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant mouse TNF-α.\u003cbr\u003e\nNo cross-reactivity: human TNF- α, β; human TNF RI, human TNF RII; Porcine TNF-α; Rat TNF-α; Mouse TNF RI, Mouse TNF RII.\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Procedures (Fig. 1)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cimg class=\"alignnone wp-image-9536 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3235-0.png\" alt=\"\" width=\"555\" height=\"113\" data-lazy-sizes=\"auto, (max-width: 555px) 100vw, 555px\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Standard Preparation (\u003c\/strong\u003e\u003cstrong\u003eFig.2)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-12325 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS3050-4.png\" alt=\"\" width=\"811\" height=\"436\" data-lazy-sizes=\"auto, (max-width: 811px) 100vw, 811px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAccurate Standard Curve (\u003c\/strong\u003e\u003cstrong\u003eFig.3 )\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-7145 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/3050-41.png\" alt=\"\" width=\"376\" height=\"268\"\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1px solid black\" width=\"1115\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse TNF-α Microplate\u003c\/td\u003e\n\u003ctd\u003eTBS3050A\u003c\/td\u003e\n\u003ctd\u003e96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for mouse TNF-α.\u003c\/td\u003e\n\u003ctd\u003eReturn unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse TNF-α Standard\u003c\/td\u003e\n\u003ctd\u003eTBS3050B\u003c\/td\u003e\n\u003ctd\u003e20 µL of Recombinant mouse TNF-α protein (100 ng\/mL).\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS3050C\u003c\/td\u003e\n\u003ctd\u003e2.2 mL of Biotin-mouse TNF-α antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003eTBS3050D\u003c\/td\u003e\n\u003ctd\u003e200 µL of Streptavidin-HRP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS3050E\u003c\/td\u003e\n\u003ctd\u003e25 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS3000T\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003eTBS3000S\u003c\/td\u003e\n\u003ctd\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003cbr\u003e\nThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRelated Products\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/tribo-mouse-il-1%ce%b2-elisa-kit\/\"\u003eTribo™ Mouse IL-1β ELISA Kit (TBS3030)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/tribo-mouse-il-2-elisa-kit\/\"\u003eTribo™ Mouse IL-2 ELISA Kit (TBS3031)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/tribo-mouse-il-4-elisa-kit\/\"\u003eTribo™ Mouse IL-4 ELISA Kit (TBS3032)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/tribo-mouse-il-6-elisa-kit\/\"\u003eTribo™ Mouse IL-6 ELISA Kit (TBS3040)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/tribo-mouse-il-10-elisa-kit\/\"\u003eTribo™ Mouse IL-10 ELISA Kit (TBS3044)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBS3050--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS3050_mouse_TNFa-05_2023-datasheet.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS3050_Mouse-TNFa-ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReferences\u003c\/h2\u003e\n\u003cp\u003eLiao, Y., Xu, F., Yan, Y., Zhou, S., Liu, N., Dou, B., Srinivasan, N., Wang, W., Zhu, X., Ye, J. \u0026amp; Xu, Y. (2025) Chronic ethanol administration exacerbates memory loss by altering N6-methyladenosine-mediated epigenetic signaling. \u003cem\u003eFrontiers in Immunology\u003c\/em\u003e, 16. \u003ca href=\"https:\/\/doi.org\/10.3389\/fimmu.2025.1455994\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.3389\/fimmu.2025.1455994\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"TriBioScience","offers":[{"title":"1x96-well plate","offer_id":45986371698767,"sku":"TBS3050-01","price":1457.0,"currency_code":"ILS","in_stock":true},{"title":"2x96-well plate","offer_id":45986371731535,"sku":"TBS3050-02","price":2553.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3050.jpg?v=1782809694","url":"https:\/\/www.danyel.co.il\/products\/tribo-mouse-tnf-elisa-kit-tbs3050-01","provider":"danyel biotech","version":"1.0","type":"link"}