{"product_id":"tribo-mouse-ngf-elisa-kit-tbs3070","title":"Tribo™ Mouse NGF ELISA Kit","description":"\u003cp\u003eNerve Growth Factor (NGF) is a small secreted protein and a member of the neurotrophin family. NGF plays important roles in the growth, maintenance, and survival of certain target sympathetic and sensory neurons. NGF is expressed by inflammatory cells and affects changes that lead to increased neural responsiveness. NGF could be a pivotal inflammatory mediator in tissue injury and inflammation or pain.\u003c\/p\u003e\n\u003cp\u003eThe Tribo™ Mouse NGF ELISA Kit is designed to detect mouse NGF levels quantitatively in cell culture supernatants and different tissue samples including skin, muscle, neural, serum, and other biological samples. The plate has been coated with sheep polyclonal antibody against mouse NGF. Any NGF present in the sample or standard will be captured. A biotin-conjugated anti-murine NGF antibody is added as a detection antibody along with streptavidin-horseradish peroxidase (HRP) and an accompanying substrate. The reaction exhibits a blue color in direct proportion to the amount of NGF in the initial sample. The reaction is terminated by the addition of a stop solution and absorbance is measured at 450nm. The concentration of NGF in the samples is calculated opposite to the standard curve.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDirect Assays:\u003c\/strong\u003e quantitative NGF in plasma and serum.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKey Features\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity:\u003c\/strong\u003e The minimum detectable dose of mouse NGF is typically less than 8.0 pg\/mL.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity:\u003c\/strong\u003e This assay recognizes recombinant and natural mouse NGF. No cross-reactivity with any other cytokine was observed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 380px;height: 279px\" border=\"1px solid black\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eReagents\u003c\/th\u003e\n\u003cth\u003eQuantity\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrecoated 96-well Plate (8x 12 strips)\u003c\/td\u003e\n\u003ctd\u003e1 Plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard\u003c\/td\u003e\n\u003ctd\u003e2x 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003e1x 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003e1x 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Diluent (10x Concentrate)\u003c\/td\u003e\n\u003ctd\u003e1x 12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate Solution\u003c\/td\u003e\n\u003ctd\u003e1x 12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e1x 12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Solution (20x Concentrate)\u003c\/td\u003e\n\u003ctd\u003e1x 50mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlate Sealers\u003c\/td\u003e\n\u003ctd\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eStore the kit at 4℃ and standard at -20℃, respectively.\u003cbr\u003e\nShelf life: 6 months after receipt.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS3070--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/03\/TBS3070_mouse_NGF_ELISA_V02_2020.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS3070_Mouse_NGF_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986372157519,"sku":"TBS3070","price":1457.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3070.jpg?v=1782809708","url":"https:\/\/www.danyel.co.il\/products\/tribo-mouse-ngf-elisa-kit-tbs3070","provider":"danyel biotech","version":"1.0","type":"link"}