{"product_id":"tribo-mouse-ifn-elisa-kit-tbs3084","title":"Tribo™ Mouse IFN γ ELISA Kit","description":"\u003cp\u003eThe Fast Mouse IFN-γ ELISA is a solid phase ELISA designed to measure mouse IFN-γ levels in cell culture supernatants, serum, and plasma. \u003cspan style=\"color: #3366ff\"\u003eThe main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg\/mL.\u003c\/span\u003e The levels of mouse IFN-γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-γ protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKit Contents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 635px;height: 406px\" border=\"1px solid black\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eReagents\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IFN- γ Microplate\u003c\/td\u003e\n\u003ctd\u003e96 well microplate (12 strips of 8 wells) coated with a\u003cbr\u003e\nCapture Antibody specific for mouse IFN- γ.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse IFN- γ Standard\u003c\/td\u003e\n\u003ctd\u003e30 µl of Recombinant mouse IFN- γ protein (100ng\/mL).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003e2.1 ml of mouse IFN- γ antibody.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003e12 ml of Streptavidin-HRP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003e15 ml of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10x WashBuffer\u003c\/td\u003e\n\u003ctd\u003e12ml of concentrated solution(10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003e 12 ml of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e 6ml of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eStore the unopened kit at 2-8 ℃.\u003c\/strong\u003e Do not use past kit expiration dates.\u003cbr\u003e\nThe kit contains sufficient materials to run an ELISA on one 96 well plate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eFig.1 Simple Assay Procedures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS3084--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-7150 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/3084-2.png\" alt=\"\" width=\"597\" height=\"182\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eFig. 2 Diagram for Mouse IFN-γ standard preparation\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-7151 size-full\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/3084-3.png\" alt=\"\" width=\"662\" height=\"348\" data-lazy-sizes=\"auto, (max-width: 662px) 100vw, 662px\"\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eFig.3 Mouse IFN-γ standard curve\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-7152 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/3084-4.png\" alt=\"\" width=\"344\" height=\"243\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS3084--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS3084_Mouse_IFN-r_elisa_v03-03_2021.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS3084_Mouse-IFN-gamma_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986372550735,"sku":"TBS3084","price":1457.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3084.jpg?v=1782809724","url":"https:\/\/www.danyel.co.il\/products\/tribo-mouse-ifn-elisa-kit-tbs3084","provider":"danyel biotech","version":"1.0","type":"link"}