{"product_id":"tribo-mouse-g-csf-elisa-kit-tbs3080","title":"Tribo™ Mouse G-CSF ELISA Kit","description":"\u003cp\u003eThe Fast Mouse G-CSF ELISA is a solid phase ELISA designed to measure mouse G-CSF levels in cell culture supernates, serum, and plasma. \u003cspan style=\"color: #3366ff\"\u003eThe main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg\/mL.\u003c\/span\u003e The levels of mouse G-CSF samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse G-CSF protein.\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Contents and \u003c\/strong\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 320px\" border=\"1px solid black\" width=\"643\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eReagents\u003c\/th\u003e\n\u003cth\u003eQuantity\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse G-CSF Capture\u003c\/td\u003e\n\u003ctd\u003e96 well microplate (12 strips of 8 wells) coated with a capture antibody against mouse G-CSF.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse G-CSF Standard\u003c\/td\u003e\n\u003ctd\u003e20 µl of Recombinant mouse G-CSF protein (100ng\/mL).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003e2.1 mL of mouse G-CSF antibody-Biotin.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003e240 µl of Streptavidin-HRP (50x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003e20 ml of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10x WashBuffer\u003c\/td\u003e\n\u003ctd\u003e12ml of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003e12 ml of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e6ml of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eFig. 1 Simple Assays Procedures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-7170 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/3080-1.png\" alt=\"\" width=\"584\" height=\"164\"\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eFig. 2 Diagram for Mouse G-CSF standard preparation\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-7171 size-full\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/3080-2.png\" alt=\"\" width=\"731\" height=\"409\" data-lazy-sizes=\"auto, (max-width: 731px) 100vw, 731px\"\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eFig. 3 Mouse G-CSF Standard Concentration\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-7172 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/3080-3.png\" alt=\"\" width=\"423\" height=\"278\" data-was-processed=\"true\"\u003e\u003cbr\u003e\n\u003c!--TBS3080--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS3080_Mouse_G-CSF_elisa_v02-04_2021.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS3080_Mouse-G-CSF_ELISA_MSDS-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986372354127,"sku":"TBS3080","price":1457.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3080.jpg?v=1782809713","url":"https:\/\/www.danyel.co.il\/products\/tribo-mouse-g-csf-elisa-kit-tbs3080","provider":"danyel biotech","version":"1.0","type":"link"}