{"product_id":"tribo-mouse-ccl2-mcp-1-elisa-kit-tbs3081","title":"Tribo™ Mouse CCL2\/MCP-1 ELISA Kit","description":"\u003cp\u003eMonocyte Chemotactic Protein-1 (MCP-1), also known as Chemokine (C-C motif) ligand 2 (CCL2), is a member of the beta (CC) subfamily of chemokines. MCP-1 is characterized as a monocyte-specific chemoattractant. Because of its target cell specificity, MCP-1 is postulated to play a pathogenic role in a variety of diseases characterized by mononuclear cell infiltration, including atherosclerosis, rheumatoid arthritis and allergic responses. In basophils, MCP-1 is highly effective as a stimulus of histamine release but has only weak chemotactic activity. Additionally, it has been shown to chemoattract CD4+ and CD8+ T lymphocytes.\u003c\/p\u003e\n\u003cp\u003eThe Mouse MCP-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse MCP-1 in different tissue plasma, serum, and other biological samples. The plate has been coated with murine MCP-1 monoclonal antibody. Any MCP-1 present in the sample or standard will be captured. A biotin-conjugated anti-murine MCP-1 antibody is added followed by streptavidin-horseradish peroxidase (HRP) and an accompanying substrate. The reaction exhibits a blue color in direct proportion to the amount of MCP-1 in the initial sample. The reaction is terminated by the addition of a stop solution, and absorbance is measured at 450 nm. The concentration of MCP-1 in the samples is calculated opposite to the standard curve.\u003cbr\u003e\nThe mouse MCP-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eApplications\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDirect Assays:\u003c\/strong\u003e quantitative MCP-1 in plasma and serum.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKey Features\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity:\u003c\/strong\u003e The minimum detectable dose of mouse MCP-1 is less than 8.0 pg\/mL typically.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity:\u003c\/strong\u003e This assay recognizes recombinant and natural mouse MCP-1. No cross-reactivity with any of the other cytokine is observed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 365px;height: 296px\" border=\"1px solid black\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eReagents\u003c\/th\u003e\n\u003cth\u003eQuantity\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrecoated 96-well Plate (8x 12 strips)\u003c\/td\u003e\n\u003ctd\u003e1 Plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard\u003c\/td\u003e\n\u003ctd\u003e2x 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003e1x 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003e1x 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Diluent (10x Concentrate)\u003c\/td\u003e\n\u003ctd\u003e1x 12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate Solution\u003c\/td\u003e\n\u003ctd\u003e1x 12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e1x 12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Solution (20x Concentrate)\u003c\/td\u003e\n\u003ctd\u003e1x 50mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlate Sealers\u003c\/td\u003e\n\u003ctd\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eStore the kit at 4℃ and standard at -20℃, respectively.\u003cbr\u003e\nShelf life: 12 months after receipt.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS3081--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/03\/TBS3081_mouse_MCP1_ELISA_V02_2020.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS3081_Mouse_CCL2MCP-1_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986372386895,"sku":"TBS3081","price":1457.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3081.jpg?v=1782809717","url":"https:\/\/www.danyel.co.il\/products\/tribo-mouse-ccl2-mcp-1-elisa-kit-tbs3081","provider":"danyel biotech","version":"1.0","type":"link"}